载体及通用引物

载体名称

P3*FLAG- CMV

pAc5.1/V5-His(_A,_B,_C)

pAcG2T

pACT

pACT2

pACT2

pACYC184(BamHI-site)

pACYCDuet-1(MCSI)

pACYCDuet-1(MCSII)

pAdEasy-1

pAdTrack-CMV

pAS2-1

pB42AD

pBABE

pBacPAK8

pBAD

pBAD/HisABC

pBBR1MCS

pBD-GAL4

pBI121

pBIND

pBK- CMV

pBluescriptIIKS(+/-)

pBluescriptIISK(+/-)

pBluescriptKS(+/-)

pBluescriptSK(+/-)

pBR322(BamHI-site)

pBR322(EcoRI/HindIII-sites)

pBR325(BamHI-site)

pBR329(BamHI-site)

pBridge(MCSI)

pBridge(MCSII)

pBS-T II

pBS

（

AMP

）

pBSR

pBudCE4.1(MCSI)

pBudCE4.1(MCSII)

pBudCE4.1/lacZ/CAT

pBV220

pCAL-c

上游引物（

5'primer

）

CMV-30(825-854)

pAc5-5

pGEX-5

T7EEV

GAL4 ADfor

p17110

PBRforBam

ACYCDuetUP1 Primer

DuetUp2

not available

not available

GAL4 Bdfor/P24990

pB42ADF

pBMN_5'

BAC1

pBAD-F

pBAD-5'

T3/M13rev

pBD-GAL4-F

35S

GAL4-Bdfor

M13F/T7

T3/M13rev

T3/M13rev

T3/M13rev

T3/M13rev

PBRforBam

pBRforEco

PBRforBam

PBRforBam

GAL4-Bdfor

not available

T3/M13rev

T7

T3

CMV-Profor,T7

EF-

1α Fwd

CMV- Profor,T7

pBV220F

T7/Tac-Profor

下 游引物（

3'primer

）

CMV-24(1129-1152)

BGH rev

not available

T3/EBVrev

pGAL4-ADrv

p12584

PBRrevBam

DuetDOWN1

T7-Terminator

not available

not available

pGAL4-BDrv/P31430

PB42ADR

BAC2

pBAD-R

T7/M13for

PBD-GAL4-R

NOS

T3,EBVrev

M13R/T3

T7/M13for

（优先用

M13F

）

T7/M13for

T7/M13for

T7/M13for

PBRrevBam

pBRrevHind

PBRrevBam

PBRrevBam

3'BD

not available

T7/M13for

T3

T7ter

c-mycrev,EBVrev

BGH

c-mycrev,EBVrev

PBV220R

T7-Terminator

pCAL-n

pCAMBIA1301(1300)

pCAMBIA1304

pCAMBIA2300

pCANTB5E

pCAT3-enhancer

pCDFDuet-1(MCSI)

pCDFDuet-1(MCSII)

pcDNA1.1

pcDNA2.1

pcDNA3

pcDNA3.1(+/-)

pcDNA3.1/His(_A,_B,_C)

pcDNA3.1/myc-His(_A,_B,_C)

pcDNA3.1/V5-His(_A,_B,_C)

pcDNA3.1/Hygro(+,-)

pcDNA3.1/Zeo(+.-)

pcDNA3.3TOPOTA

pcDNA4/HisMax(_A,_B,_C)

pcDNA4/HisMax-TOPO

pcDNA4/HisMax-TOPO/lacZ

pcDNA4/myc-His(_A,_B,_C)

pcDNA4/TO

pcDNA4/V5-His(_A,_B,_C)

pcDNA4-TET/ON/AMP+

pcDNA5/FRT

pcDNA6/myc- His(_A,_B,_C)

pcDNA6/V5-His(_A,_B,_C)

pCEP4

pCI

pCI-neo

pCMS-EGFP

pCMS-EGFP

pCMV-3Tag-4A

pCMV5

pCMV6

pCMV6-XL4

pCMV-HA

pCMV-MYC(AMP+)

pCMVmyc/nuc

pcmv- scriptEXVector

pCMV-Sport6

pCOLADuet-1(MCSI)

T7/Tac- Profor

pCAMBIA1301-5'(ECORI)/M13R

pCAMBIA1301-5'(ECORI)

M13R(-48)

S1

RVP3

ACYCDuetUP1 Primer

DuetUP2

CMV-Profor,T7

M13for/T7

T7/CMV-Profor

pcDNA3.1F/T7/CMV-Profor

T7/CMV-Profor

T7/CMV- Profor

T7/CMV-Profor

T7/CMV-Profor

T7

CMV-Profor

Xpressfor

Xpressfor

Xpressfor

T7,CMV-Profor

TREfor/CMV- Profor

CMV-Profor,T7

CMV- Profor

T7/CMV-Profor

CMV- Profor,T7

CMV-Profor,T7

pCEP-F/CMV-Profor

T7

< br>（

17BASE

）

（

17BASE

）< /p>

T7

T7

T3 /PFLAG- CMV-F

pCMV5F

CMV-Profor

CMV-Profor/T7

PCMV-F,CMV-Profor,TREfor

PCMV-F

CMV-Profor

T3

T7/M13for

ACYCDuetUP1 Primer

T7-Terminator

pCAMBIA1301-3'(HindIII)

pCAMBIA1301-3'(HindIII)

M13F(-47)/M13F(-49)

S6

此载体无反向引物

DuetDOWN1

T7TER

pCDM8-3

M13rev/Tac-Profor

BGH rev/SP6

BGH rev

BGH rev

BGH rev/c-mycrev

BGH rev/V5rev

BGH rev/V5rev

BGHrev

TKPAreverse

BGH rev

BGH rev

BGH rev

BGH rev,c-mycrev

BGH rev

BGH rev,V5rev

BGH rev

BGH rev,c-mycrev

BGH rev,V5rev

EBVrev

EBVrev

EBVrev,T3

T3

EBVrev,T3

T7

PCMV5R

pCMV6-3

、

HGHrev

pCMV6-3

、

HGHrev

PCMV-R

，

EBVrev

PCMV-R, EBVrev

BGH rev,c-mycrev

T7

SP6/M13rev

DuetDOWN1

pCOLADuet-1(MCSII)

pCR2.1

pCR2.1-TOPO

pCR3.1

pCR3.1-Uni

pCR4

pCR4-Blunt

pCR4-TOPO

pCR-Blunt

pCR-BluntII

pCR-BluntII-TOPO

pCRII

pCRII-TOPO

pCR-ScriptSK(+)

pCR-XL-TOPO

pCS2+(MCSI)

pCS2+(MCSII)

pDB_GAL4cam

pDB_leu

pDEST22

pDEST15

pDEST20

pDEST26

pDisplay

pDNR-LIB(MCS_A)

pDonar

pDONR201

pDONR207

pDONR223

pDrive

pDsRED1-C1

pDsRed1-N1

pDsRed2-N1

pDsRed2-C1

pDsRED2-C1(KAN+)

pDsRed- Express

pDSRED-monomer-N1(KAN+)

pDSRED-N1(KAN+)

pEASY-Blunt

pEASY-BluntSimple

pEASY-T1 Simple

DuetUP2

T7,M13for

M13for,T7

T7

T7

T3/M13rev

T3/M13rev

M13rev,T3

M13rev

M13rev/SP6

M13rev/SP6

M13rev/SP6

M13rev/SP6

T7/M13for

M13rev

SP6

T3

T7P

PDBLeu-5

DEST22F

pGEX-5

pGEX-5

TREfor/CMV-Profor

T7/CMV-Profor

M13for,T7

M13F

PDONR-F

PDONR-F

M13F

T7/M13rev

(DsRed1-N-f)

CMV-Profor

(DsRed1-N-f)

(DsRed1-N-f)

pDsRED-ex-C1-F

dsRed1_N_primer/CMV-F

dsRed1_N_primer

pEGFP-N-

5’

M13F

M13F

M13F

T7TER

M13rev

M13rev

BGH rev

BGH rev

T7/M13for

T7/M13for

M13for,T7

T7,M13for

T7/M13for

M13for/T7

T7/M13for

M13for/T7

T3/M13rev

T7,M13for

EBVrev,T7?

pSG5-3

PDBLeu-3

DEST22R

T7-Terminator

pFastBac-3

T7/M13for

c-mycrev

pSG5-3,M13R

M13R

PDONR-R

PDONR-R

M13R

SP6/M13for

DsRed1-C-r

RFP- Nrev,CMV-R?

DsRed1-C-r

DsRed1-C-r

pEGFP-N-

3’

dsRed1_C_primer/CMV-R

pDSRED-N-R

T7/ M13R

T7/ M13R

（距离插入位点合

适，优先安排）

M13R

（ 距离插入位点合适，

优先安排）

pEASY-T1

pEASY-T3

pECFP-N(C)

pEF/myc/cyto/ER/mito/nuc

pEF1(4,6/myc-His)

pEF1(4,6/V5-His)

pEF1(4;6/His_A;_B;_C)

pEF5/FRT/V5-D(-TOPO)

pEGFP

pEGFP-1

pEGFP-C

pEGFP-C1

pEGFP-C2

pEGFP-C3

pEGFP-F

pEGFP-N

pEGFP-N1

pEGFP-N2

pEGFP-N3

pENTR

pET-11(-a,-b,-c,-d)

pET-12(-a,-b,-c)

pET-14b

pET-15b

pET-16b

pET-17b

pET-17xb

pET-19b

pET-20b(+)

pET-21(-a,-b,-c,-d)(+)

pET-22b(+)

pET-23(-a,-b,-c,-d)(+)

pET-24(-a,-b,-c,-d)(+)

pET-25b(+)

pET-26b(+)

pET-27b(+)

pET-28a(-b,-c)(+)

pET-29a(-b,-c)(+)

pET-3(-a,-b,-c,-d)

pET-30_Ek/LIC

pET-30_Xa/LIC

pET-30a(-b,-c)(+)

pET-31b(+)

M13F/T7

M13F/T7

PECFP-N-5.

pEF-5

T7/pEF-5

T7/pEF-5

T7/Xpressfor

T7/pEF-5

M13rev

not available

PEGFP-C-

5′

EGFP-Cfor

EGFP-Cfor

EGFP-Cfor

EGFP-Cfor

PEGFP-N-

5′

CMV-Profor

CMV-Profor

CMV-Profor

M13F

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

/T7

T7

T7

T7

/T7

not available

M13R

M13R/SP6

PECFP-N-3,

Pcdna3.1R/BGH rev

Pcdna3.1R/BGH rev

Pcdna3.1R/BGH rev

Pcdna3.1R/BGH rev

Pcdna3.1R/BGH rev

EGFP-Nrev

EGFP-Nrev/ PEGFP-N-3

′

PEGFP-C-

3′

SV40-pArev

SV40-pArev

SV40-pArev

SV40-pArev

PEGFP-N-3

′

EGFP-Nrev

EGFP-Nrev

EGFP-Nrev

M13R

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

pET-32_Ek/LIC

pET-32_XA/LIC

pET-32a(-b,-c)(+)

pET-33b(+)

pET-34b(+)

pET-35b(+)

pET-36b(+)

pET-37b(+)

pET-38b(+)

pET-39b(+)

pET-40b(+)

pET-41_Ek/LIC

pET-41a(-b,-c)(+)

pET-42a(-b,-c)(+)

pET-43.1a(-b,-c)(+)

pET-43.1a_EK/LIC

pET-44a(-b,-c)(+)

pET-44a_EK/LIC

pET-45b(+)

pET-46_Ek/LIC

pET-5(-a,-b,-c)

pET-52b(+)

pET-7

pET-9(-a,-b,-c,-d)

pETcoco-1

pETDuet-1(MCSI)

pETDuet-1(MCSII)

pET-T4

pEYFP-C1

pEYFP-N1

pFLAG-ATS

pFLAG-CTC

pFLAG1

pFLAG-CMV(-2)

pFLAG-CMV1

pGAD424

pGADGH

pGADT7

pGADT7-Rec

pGADT-T

pGAD10

pGAPZa-A

pGBKT7

TRXfor

TRXfor

/TRXfor

T7

not available

GST-Cfor

/pGEX-5

/pGEX-5

T7

T7

T7

T7

T7

T7

T7

pETUP1

DuetUp2

T7

pEGFP-C-

5’

pEGFP-N-

5’,CMV

-Profor

N26for

N26for

N26for

CMV-30

；

CMV-Profor

CMV-30

GAL4_ADfor

GAL4_ADfor

T7/GAL4_ADfor

T7/GAL4_ADfor

T7

GAL4_ADfor

a-FACTOR

T7

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

coliDOWN

not available

T7-Terminator

not available

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

DuetDOWN1

T7-Terminator

T7-Terminator

pEGFP-C-3'

pEGFP-N-

3’

pTrcHis- rv

；

C24rev

pTrcHis-rv

；

C24rev

pTrcHis-rv

；

C24rev

CMV-24

CMV-24

3'AD

3'AD

3'AD

3'AD

3,BD

3'AD

3'AOX

3,BD