-热情
pEASY-Blunt Simple Cloning Vector Complete
sequence
AGCGCCCAATACGCAAAC
CGCCTCTCCCCGCGCGTTGGCCGA
TTCATTAATGCAGCT
G
GCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGC
GCAACGCAATTAATGTGAG
TTAGCTCACTCATTAGGCAC
CCCAGGCTTTACACTTTA
TGCTTCCGGCTCGTATGTTGT
G
TGGAATTGTGAGCGGA
TAACAATTTCA
CACAGGAAACAGCTATGACCA
TGATTACGCC
< br>AAGCTGCCCTTAAGGGCAGCTTCAA
TTCGCCCTATA
GTGAGTCGTATTACAATTCACTG
GCCGTCGTTTTACAAC
GTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC
< br>TTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCC CGCACCGA
TCG
CCCTTCCCAACAGTTGC
GCAGCCTGAA
TGGCGAA
TGGACGCGCCC
TGTAGCGGCGCAT
TAAGCGCGGCGGGTGTGGTGGTTACG
CGCAGCGTGACCGCTACACTTGCCAGCGCCCT
AGCGCCC
GCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCC
CC
GTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCG
ATTTAGTGCTTTACGGCACCTC
GACCCCAAAAAACTTGA
TTAGGGTGA
TGGTTCACGTAGTGGGCCA
TCGCCCTGATAGA
CGGTTTTTCGCCCTTTGACGTTGGAG
TCCACGTTCTTTAATAGTGGACTCTTGTTCCAA
ACTGGA
ACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGA
TTTTGCCG
ATTTCGGCCTATTGGTTAAAAAAT
GAGCTGA
TTTAACAAAAA
TTTAACGCGA
ATTTTAA
CAAAATTCAGGGCGCAAGGGCTGCTAAAGGAA
GCGGAACACGTAGAAAGCCAGTCCG
CAGAAACGGTGCTG
ACCCCGGA
TGAATGTCAGCTACTGGGCTATCTGGACAAG
GGAA
AACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAG
TGGGCTTACATGGCGA
TAGCTA
GACTGG
GCGGTTTTA
TGGACAGCAAGCGAACCGGAA
TTGCCAGCTGGGGCGCCCTCTG
GTAAGGTTGGGAAGC
CCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTG
ATGGCGCAGGGGA
TCAAGA
TCTGA
TCAAGAGACAGGATGAGGATCGTTTCGCA
TGA
TT
GAACAAGATGGATTGCACGCAG
GTTCTCCGGCCGCTTGGGTGGAGAGGCTA
TTCGGCT
ATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCG
TGTTCCGGCTGTCAGC
GCAGGGGCGCCCGGTTCTTTTTG
TCAAGACCGACCTGTCCGGTGCCCTGAATGAACTG
CAGG
ACGAGGCAGCGCGGCTA
TCGTGGCTGGCCACGACGGGCG
TTCCTTGCGCAGCT
GTGCTCGACGTTGTCACTGAAGCGG
GAAGGGACTGGCTGCTATTGGGCGAAGTGCCG
GGGCAGG
ATCTCCTGTCATCCCACCTTGCTCCTGCCGAGAAAGTA
T
CCATCATGGCTGA
TGCAATGCGGCGGCTGCATACGCTTG
ATCCGGCTACCTGCCCATTCGACCACCAAGCG
AAACATC
GCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGAT
G
ATCTGGACGAAGAGCA
TCAGGGGCTCG
CGCCAGCCGAACTGTTCGCCAGGCTCAAGG
CGCGCA
TGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGA
TGCCTGCTTGCCGAA
TA
TCATG
GTGGAAAA
TGGCCGCTTTTCTGGATTCATCGACTGTGGC
CGGCTGGGTGTGG
CGGACCGCTATCAGGACATAGCGTTG
GCTACCCGTGATATTGCTGAAGAGCTTGGCGG
CGAATGG
GCTGACCGCTTCCTCGTGCTTTACGGTA
TCGCCGCTCCC
GA
TTCGCAGCGCA
TCGCCTTCTATCGCC
TTCTTGACGAGTTCTTCTGAATTGAAAAAGGAAGAGTATGAGTA
TTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGC
TCACCCAGAAAC
GCTGGTGAAAGTAAAAGA
TGCTGAAGATCAGTTGGGTGC
ACGAGT
GGGTTACATCGAACTGGATCTCAACAGCGGTAA
GATCCTTGAGAGTTTTCGCCCCGAA
GAACGTTTTCCAAT
GA
TGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATT
ATCCCGT
ATTGACGCCGGGCAAGAGCAACTCGGTCGCCG
CATACACTA
TTCTCAGAATGACTTGG
TT
GAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAG
AGAATT
ATGCAGTGCTGCCA
TAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACGA
TC
GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGA
TCATGTAACTCG
CCTTGATCGTTGGGAACCGGAG
CTGAA
TGAAGCCA
TACCAAACGACGAGC
GTGACAC
CACGA
TGCCTGTAGCAA
TGGCAACAACGTTGCGCAAACTA
TTAACTGGC
GAACTACTT
ACTCTAGCTTCCCGGCAACAATTAA
TAGACTGGATGGAGGCGGA
TAAAGTTGCAGGAC
CACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTG
CTGA
TAAA
TCTGGAGCCGGT
< br>GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAA GCCCTCCCGTA
TCGTAGTTATCTACACGACGGGGAGTCA
GGCAACTATGGATGAACGAAATAGACAGA
T
CGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCA AGTTTACTCA
T
ATATACTTTAGATTGAT
TTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCT
TTTTGATAATCTCA
TGACCAAAATCCCTTAACGT
GAGTTTTCGTTCCACTGAGCGTCAG
ACCCCGTAGAAAAG
ATCAAAGGATCTTCTTGAGA
TCCTTTTTTTCTGCGCGTA
A
TCTGC
TGCTTGCAAACAAAAAAACCAC
CGCTACCAGCGGTGGTTTGTTTGCCGGA
TCAAGAGC
TACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGC
AGATACCAAATACTGTT
CTTCTAGTGTAGCCGTAGTTAG
GCCACCACTTCAAGAACTCTGTAGCACCGCCTACATA
CC
TCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCG
TGTCTTA
CCGGGTTGGACTCAAGACGATAGTTACCGGA<
/p>
TAAGGCGCAGCGGTCGGGCTGAACGG
GGG
GTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGA
TACCT
ACAGCGTGAGCTA
TGAGAAAGCG
CCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTA
TCCGG
TAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGA
A
ACGCCTGGTATCTTTA
TAGTCCTGTCG
GGTTTCGCCACCTCTGACTTGAGCGTCGATTTT
TGTGA<
/p>
TGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCA
ACGCGGCCTTTTT
ACGGTTCCTGGCCTTTTGCTGGCCTT
TTGCTCACATGTTCTTTCCTGCGTTA
TCCCCTGA
TTCTGTGGATAACCGTA
TTACCGCCTTTGAGTG
AGCTGA
TACCGCTCGCCGCAGCCGAA
CG
ACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG
Lac
Z
??
fragment:
bases 1-449
M13 reverse priming site:
bases 205-221
T7 promoter/priming site:
bases 265-282
M13 Forward (-20) priming
site: bases 290-305
f1 origin: bases
447-884
Kanamycin resistance ORF: bases
1218-2012
Ampicillin resistance ORF:
bases 2030-2890
pUC origin: bases
3035-3708
pEASY-Blunt Simple Cloning
Vector
Restriction
Map
NEWMOL
Cutters
(349)
rar
25, 46, 72, 123,
127,
145, 152, 159, 166,
201
245, 256, 262, 289,
303,
344, 353, 369,
414, 514
536,
540, 545, 549,
554, 558, 562, 571,
579, 593
607, 611, 619, 625,
632, 643, 658, 675,
707, 716
736, 751, 757, 775,
786,
795, 799, 804,
815, 824
854,
865, 876, 887,
1024, 1035, 1063,
1106, 1147, 1151
1215, 1250,
1261,
1278, 1313, 1329,
1357, 1361, 1444,
1461
1524, 1539, 1543,
1596,
1617, 1657,
1674, 1713, 1731,
1740
1770, 1796, 1826,
1836, 1914, 1937,
1941,
1947, 1963,
1970
1985, 1995,
2007,
2040, 2047, 2060,
2064, 2068, 2072,
2081
2087, 2094, 2100,
2177,
2195, 2203,
2221, 2238, 2247,
2297
2317, 2329, 2341,
2363, 2434, 2438,
2475,
2479, 2505,
2510
2621, 2625,
2631,
2684, 2692, 2699,
2714, 2751, 2791,
2872
2907, 2911, 2921,
2932,
2939, 2944,
2951, 2975, 2987,
3003
3014, 3020, 3060,
3072, 3076, 3097,
3132,
3136, 3159,
3163
3181, 3210,
3241,
3250, 3272, 3276,
3326, 3340, 3391,
3409
3473, 3545, 3569,
3587,
3596, 3602,
3614, 3625, 3670,
3680
3688, 3699, 3705,
3713, 3717, 3736,
3760,
3780, 18, 83
96, 116, 181, 188,
211, 237, 248, 275,
323, 392
436, 443, 471, 590,
597,
652, 727, 761,
767, 809
840,
847, 860, 873,
883, 897, 907, 911,
917, 926
941, 962, 989, 993,
1001, 1007, 1012,
1076,
1080, 1086
1113, 1121, 1131,
1136, 1160, 1184,
1195,
1207, 1227,
1231
1269, 1368,
1391,
1395, 1407, 1476,
1483, 1506, 1551,
1555
1627, 1631, 1644,
1648, 1665, 1698,
1725,
1743, 1764,
1802
1815, 1908,
1923,
2004, 2014, 2019,
2023, 2027, 2035,
2108
2119, 2125, 2135,
2154,
2168, 2189,
2198, 2213, 2233,
2243
2289, 2323, 2336,
2354, 2383, 2387,
2413,
2454, 2461,
2465
2522, 2528,
2533,
2537, 2548, 2555,
2600, 2615, 2660,
2669
2726, 2733, 2741,
2786,
2821, 2840,
2844, 2861, 2882,
2904
2935, 2954, 2968,
2996, 3011, 3027,
3044,
3053, 3066,
3102
3106, 3110,
3147,
3169, 3189, 3202,
3245, 3317, 3343,
3362
3381, 3413, 3417,
3431,
3436, 3452,
3459, 3463, 3479,
3484
3488, 3508, 3522,
3528, 3538, 3542,
3555,
3605, 3640,
3649
3764, 3768,
3792,
3800, 3809, 3817,
3821, 3825, 3830
(299)
gc
4, 15, 22, 32, 40, 54,
57, 61, 86, 90
97, 104, 121,
134,
143, 157, 164, 189,
216, 233
238, 241, 251, 254,
264, 295, 332, 350,
355, 358
373, 377, 381, 389,
397,
401, 410, 426,
431, 440
450,
460, 463, 472,
477, 494, 499, 507,
516, 520
528, 534, 543, 564,
573, 577, 591, 605,
630, 638
686, 692, 712, 818,
828,
848, 870, 892,
900, 903
913,
929, 937, 948,
969, 977, 998, 1002,
1015, 1022
1026, 1033, 1042,
1048, 1058, 1073,
1077,
1090, 1094,
1100
1123, 1128,
1145,
1153, 1156, 1171,
1218, 1240, 1244,
1256
1259, 1273, 1281,
1292,
1308, 1311,
1319, 1322, 1333,
1340
1348, 1385, 1399,
1409, 1412, 1417,
1426,
1430, 1440,
1449
1454, 1459,
1478,
1492, 1495, 1503,
1510, 1516, 1541,
1547
1568, 1574,
1579,
1582, 1585, 1588,
1594, 1604, 1611,
1628
1638, 1645, 1649,
1667,
1702, 1711,
1715, 1721, 1733,
1738
1745, 1749, 1753,
1761, 1785, 1790,
1794,
1798, 1821,
1824
1848, 1852,
1862,
1869, 1883, 1889,
1904, 1912, 1917,
1920
1928, 1935, 1945,
1958,
1961, 1972,
1975, 1982, 1992,
2056
2074, 2077, 2084,
2098, 2112, 2131,
2149,
2183, 2207,
2234
2250, 2258,
2281,
2286, 2292, 2303,
2306, 2358, 2372,
2393
2398, 2401, 2425,
2428,
2466, 2473,
2481, 2507, 2529,
2539
2556, 2571, 2578,
2584, 2595, 2612,
2629,
2637, 2662,
2674
2688, 2695,
2704,
2708, 2719, 2734,
2742, 2753, 2764,
2767
2775, 2787, 2827,
2857,
2869, 2883,
3028, 3081, 3092,
3095
(256)
cg
3099, 3117, 3124,
3138,
3150, 3179,
3185, 3190, 3222,
3233
3256, 3261, 3274,
3279, 3298, 3301,
3304,
3311, 3364,
3369
3377, 3395,
3402,
3407, 3414, 3447,
3453, 3464, 3471,
3490
3509, 3512, 3531,
3543,
3559, 3590,
3606, 3623, 3636,
3641
3655, 3659, 3664,
3667, 3685, 3692,
3696,
3703, 3721,
3757
3769, 3778,
3782,
3785, 3788, 3801,
3806, 3818, 3826
5, 14, 21,
31, 35, 42,
64, 76, 87, 98
103, 162, 167, 190,
232,
263, 278, 297,
300, 310
313,
333, 349, 372,
382, 390, 400, 405,
409, 427
441, 449, 461, 464,
473, 478, 493, 500,
506, 521
529, 533, 542, 563,
568,
572, 575, 585,
600, 621
636,
644, 678, 691,
703, 711, 721, 733,
787, 820
826, 869, 893, 914,
921, 936, 944, 956,
997,
1003
1043, 1059, 1078,
1083, 1101, 1155,
1172, 1212, 1217,
1243
1254, 1258, 1279,
1306,
1321, 1324,
1331, 1341, 1349,
1353
1372, 1381, 1405,
1413, 1422, 1434,
1437,
1441, 1450,
1462
1465, 1479,
1504,
1512, 1549, 1580,
1583, 1593, 1602,
1618
1629, 1637, 1642,
1646,
1652, 1658,
1669, 1678, 1696,
1714
1723, 1732, 1746,
1756, 1759, 1762,
1771,
1774, 1786,
1800
1823, 1840,
1850,
1863, 1868, 1884,
1895, 1918, 1921,
1934
1942, 1951, 1957,
1960,
1966, 1971,
1976, 1981, 1991,
2002
2050, 2055, 2075,
2111, 2152, 2166,
2184,
2206, 2211,
2218
2259, 2272,
2280,
2283, 2298, 2302,
2305, 2366, 2426,
2447
2451, 2459, 2472,
2506,
2515, 2525,
2550, 2553, 2557,
2567
2591, 2596, 2613,
(254)
rgcb
2635, 2663,
2689,
2693, 2702, 2736,
2743
2752, 2794, 2799,
2813,
2816, 2842,
2856, 3007, 3017,
3029
3039, 3082, 3116,
3125, 3140, 3167,
3191,
3224, 3260,
3273
3312, 3320,
3330,
3346, 3356, 3365,
3370, 3374, 3383,
3392
3415, 3419, 3429,
3448,
3465, 3470,
3477, 3491, 3503,
3510
3519, 3532, 3536,
3558, 3582, 3589,
3607,
3610, 3626,
3637
3654, 3663,
3676,
3722, 3748, 3756,
3777, 3781, 3784,
3790
3794, 3798, 3802,
3807,
3819, 3827
7, 23, 41, 58, 122,
144, 165, 217, 234,
239
255, 265, 296, 351,
374,
398, 411, 432,
453, 508
523,
531, 544, 565,
574, 592, 606, 687,
693, 713
829, 849, 901, 930,
970, 978, 1023, 1034,
1049,
1095
1103, 1124, 1146,
1157,
1257, 1274,
1282, 1309, 1323,
1334
1351, 1418, 1427,
1455, 1493, 1569,
1586,
1595, 1605,
1668
1712, 1718,
1734,
1739, 1822, 1849,
1870, 1890, 1913,
1929
1936, 1959, 1983,
1993,
2057, 2113,
2208, 2282, 2304,
2429
2467, 2474, 2508,
2530, 2540, 2630,
2691,
2696, 2705,
2735
2776, 2788,
2858,
3118, 3151, 3180,
3223, 3234, 3262,
3275
3299, 3378, 3403,
3408,
3454, 3467,
3472, 3544, 3560,
3591
3642, 3656, 3668,
3686, 3697, 3758,
3770,
3779, 3789, 5
41, 58, 87, 98, 122,
144, 165, 190, 217,
239
255, 296, 333, 378,
390,
398, 432, 441,
461, 473
478,
500, 521, 529,
578, 592, 606, 687,
829, 849
893, 901, 914, 930,
970, 978, 1003, 1023,
1034,
1043
1049, 1059, 1078,
1095,
1101, 1124,
1146, 1172, 1257,
1274
(254)
rgcb
1282, 1309,
1334,
1341, 1349, 1413,
1418, 1427, 1441,
1455
1479, 1493, 1504,
1569,
1583, 1605,
1629, 1646, 1668,
1712
1722, 1739, 1746,
1762, 1786, 1822,
1849,
1863, 1884,
1890
1913, 1918,
1929,
1976, 2184, 2259,
2429, 2467, 2530,
2540
2557, 2613, 2630,
2663,
2696, 2705,
2735, 2743, 2776,
2788
3029, 3125, 3151,
3180, 3191, 3223,
3234,
3299, 3312,
3365
3370, 3378,
3403,
3408, 3415, 3448,
3454, 3465, 3491,
3510
3532, 3544, 3607,
3637,
3642, 3668,
3686, 3697, 3770,
3789
3802, 3807, 3819,
3827
7, 23, 41, 58, 122,
144, 165, 217, 234,
239
255, 265, 296, 351,
374,
398, 411, 432,
453, 508
523,
531, 544, 565,
574, 592, 606, 687,
693, 713
829, 849, 901, 930,
970, 978, 1023, 1034,
1049,
1095
1103, 1124, 1146,
1157,
1257, 1274,
1282, 1309, 1323,
1334
1351, 1418, 1427,
1455, 1493, 1569,
1586,
1595, 1605,
1668
1712, 1718,
1734,
1739, 1822, 1849,
1870, 1890, 1913,
1929
1936, 1959, 1983,
1993,
2057, 2113,
2208, 2282, 2304,
2429
2467, 2474, 2508,
2530, 2540, 2630,
2691,
2696, 2705,
2735
2776, 2788,
2858,
3118, 3151, 3180,
3223, 3234, 3262,
3275
3299, 3378, 3403,
3408,
3454, 3467,
3472, 3544, 3560,
3591
3642, 3656, 3668,
3686, 3697, 3758,
3770,
3779, 3789, 5
41, 58, 87, 98, 122,
144, 165, 190, 217,
239
255, 296, 333, 378,
390,
398, 432, 441,
461, 473
478,
500, 521, 529,
578, 592, 606, 687,
829, 849
893, 901, 914, 930,
970, 978, 1003, 1023,
(96)
cac
1034, 1043
1049, 1059, 1078,
1095, 1101, 1124,
1146,
1172, 1257,
1274
1282, 1309,
1334,
1341, 1349, 1413,
1418, 1427, 1441,
1455
1479, 1493, 1504,
1569,
1583, 1605,
1629, 1646, 1668,
1712
1722, 1739, 1746,
1762, 1786, 1822,
1849,
1863, 1884,
1890
1913, 1918,
1929,
1976, 2184, 2259,
2429, 2467, 2530,
2540
2557, 2613, 2630,
2663,
2696, 2705,
2735, 2743, 2776,
2788
3029, 3125, 3151,
3180, 3191, 3223,
3234,
3299, 3312,
3365
3370, 3378,
3403,
3408, 3415, 3448,
3454, 3465, 3491,
3510
3532, 3544, 3607,
3637,
3642, 3668,
3686, 3697, 3770,
3789
3802, 3807, 3819,
3827
94, 114, 176, 186,
273, 315, 482, 487,
502, 629
668, 683, 746, 947,
1030,
1266, 1326,
CviJI (75)
rgcy
1384, 1424,
1458
1509, 1776, 1812,
1846,
1858, 1897,
1944, 2052, 2117,
2148
2156, 2256, 2397,
2415, 2559, 2739,
2745,
2868, 2966,
3009
3128, 3218,
3296,
3309, 3322, 3394,
3450, 3619, 3740,
3766
3815, 63, 125, 136,
150,
204, 291, 360,
403, 512
567,
640, 677, 732,
773, 920, 1242, 1294,
1433, 1472
1536, 1623, 1651,
2102, 2151, 2236,
2312,
2343, 2350,
2422
2483, 2563,
2682,
2769, 2812, 2874,
3023, 3114, 3236,
3258
3397, 3427, 3469,
3535,
3593, 3707
41, 58, 122, 144, 165,
217, 239, 255, 296,
378
398, 432, 578, 592,
606,
687, 829, 849,
901, 930
970,
978, 1023, 1034,
1049, 1095, 1124,
1146, 1257, 1274
1282, 1309,
1334,
1418, 1427, 1455,
1493, 1569, 1586,
1605
1668, 1712, 1722,
1739,
1822, 1849,
(75)
(56)
rgcy
gcchr
1890, 1913, 1929,
2429
2467, 2530, 2540,
2630,
2696, 2705,
2735, 2776, 2788,
3151
3180, 3223, 3234,
3299, 3378, 3403,
3408,
3454, 3544,
3642
3668, 3686,
3697,
3770, 3789
41, 58,
122, 144, 165,
217, 239, 255, 296,
378
398, 432, 578, 592,
606, 687, 829, 849,
901, 930
970, 978, 1023, 1034,
1049,
1095, 1124,
1146, 1257, 1274
1282, 1309, 1334,
1418,
1427, 1455,
1493, 1569, 1586,
1605
1668, 1712, 1722,
1739, 1822, 1849,
1890,
1913, 1929,
2429
2467, 2530,
2540,
2630, 2696, 2705,
2735, 2776, 2788,
3151
3180, 3223, 3234,
3299,
3378, 3403,
3408, 3454, 3544,
3642
3668, 3686, 3697,
3770, 3789
40, 61, 90, 134,
143,
295, 332, 381, 686,
977
1033, 1058, 1292,
1430,
1440, 1492,
1503, 1738, 1889,
1917
1928, 2286, 2612,
2708,
2827, 3179,
3233, 3377, 3685,
3696
9, 236, 376, 400, 434,
AciI (44)
BthCI (34)
ccgc
gcngc
519, 533, 831, 932,
1093
1159, 1353, 1614,
1720,
1736, 1756,
1793, 2431, 2574,
2778
3264, 3307, 3405,
3562, 3644, 3658
88, 191,
462, 476,
915, 1060, 1416,
1480, 1581, 1864
1919, 2076,
2185,
2262, 2427, 2664,
2755, 3126, 3371,
3492
3511, 3638, 3666,
3828, 22,
32, 401,
507, 534, 937
1156,
1259, 1322,
1824, 1869, 1935,
1961, 2306, 2473,
3117
3261, 3757, 3778,
3785
57, 241, 254, 358,
431, 463,
477, 499,
903, 1156
1259,
1311, 1322,
1412, 1454, 1495,
1582, 1588, 1824,
1920
1961, 1975, 2077,
2306,
2401, 2428,
2767, 3095, 3301,
3369
3512, 3667, 3785,
3806
Fnu4HI (34)
HhaI (30)
HinP1I (30)
(30)
gcngc
gcgc
gcgc
gcgc
57, 241, 254, 358,
431, 463,
477, 499,
903, 1156
1259,
1311, 1322,
1412, 1454, 1495,
1582, 1588, 1824,
1920
1961, 1975, 2077,
2306,
2401, 2428,
2767, 3095, 3301,
3369
3512, 3667, 3785,
3806
6, 34, 99, 428, 452,
465, 474, 496, 522,
530
894, 1004, 1102,
1173, 1342,
1350,
1414, 1451, 1717,
1747
1977, 2260, 2597,
2690,
3083, 3192,
3366, 3466, 3533,
3803
6, 34, 99, 428, 452,
465, 474, 496, 522,
530
894, 1004, 1102,
1173, 1342,
1350,
1414, 1451, 1717,
1747
1977, 2260, 2597,
2690,
3083, 3192,
3366, 3466, 3533,
3803
6, 34, 99, 428, 452,
465, 474, 496, 522,
530
894, 1004, 1102,
1173, 1342,
1350,
1414, 1451, 1717,
1747
1977, 2260, 2597,
2690,
3083, 3192,
1I (30)
II (30)
Sth132I (28)
BstKTI (24)
ChaI (24)
gcgc
gcgc
cccg
gatc
gatc
3366, 3466, 3533,
3803
6, 34, 99, 428, 452,
465,
474, 496, 522,
530
894,
1004, 1102,
1173, 1342, 1350,
1414, 1451, 1717,
1747
1977, 2260, 2597,
2690,
3083, 3192,
3366, 3466, 3533,
3803
6, 34, 99, 428, 452,
465, 474, 496, 522,
530
894, 1004, 1102,
1173, 1342,
1350,
1414, 1451, 1717,
1747
1977, 2260, 2597,
2690,
3083, 3192,
3366, 3466, 3533,
3803
89, 480, 602, 1439,
1481, 1514, 2285,
2818,
3332, 3376
3385, 3584, 31, 76,
400, 533, 585, 956,
1353,
1756
1895, 1966, 2211,
2272,
2635, 2794,
3039, 3477
408,
1164, 1181,
1187, 1192, 1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
DpnI (24)
(24)
2I (24)
(24)
gatc
gatc
gatc
gatc
1187, 1192,
1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192, 1211,
1522,
1600, 1681,
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192,
1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192, 1211,
1522,
1600, 1681,
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192,
1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
(24)
6I (24)
(24)
(24)
gatc
gatc
gatc
gatc
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192,
1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192, 1211,
1522,
1600, 1681,
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192,
1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192, 1211,
1522,
1600, 1681,
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
(24)
67Dam (24)
m (24)
1Dam (24)
1Dam (24)
gatc
gatc
gatc
gatc
gatc
3144
408,
1164, 1181,
1187, 1192, 1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192, 1211,
1522,
1600, 1681,
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192,
1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192, 1211,
1522,
1600, 1681,
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192,
1211,
1522, 1600, 1681,
2Dam (24)
4Dam (24)
I (24)
m (24)
gatc
gatc
gatc
gatc
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192,
1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192, 1211,
1522,
1600, 1681,
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192,
1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192, 1211,
1522,
1600, 1681,
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
1Dam (24)
99VI (24)
I (24)
IA (24)
IB (24)
gatc
gatc
gatc
gatc
gatc
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192, 1211,
1522,
1600, 1681,
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192,
1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192, 1211,
1522,
1600, 1681,
1690
1768, 2139,
2175,
2192, 2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,
1187, 1192,
1211,
1522, 1600, 1681,
1690
1768, 2139, 2175,
2192,
2450, 2496,
2514, 2855, 2960,
2972
3050, 3058, 3069,
3144
408, 1164, 1181,