-
荧光素名称
1,5 IAEDANS
1,8-ANS
4-Methylumbelliferone
5-carboxy-2,7-dichlorofluorescein
5-Carboxyfluorescein (5-FAM)
5-Carboxynapthofluorescein (pH 10)
5-Carboxytetramethylrhodamine (5-TAMRA)
5-FAM (5-Carboxyfluorescein)
5-HAT (HydroxyTryptamine)
5-Hydroxy Tryptamine (HAT)
5-ROX (carboxy-X-rhodamine)
5-TAMRA (5-Carboxytetramethylrhodamine)
6-Carboxyrhodamine 6G
6-CR
6G
6-JOE
7-Amino-4-methylcoumarin
7-Aminoactinomycin D (7-AAD)
7-Hydroxy-4-methylcoumarin
9-Amino-6-chloro-2-methoxyacridine
ABQ
Acid Fuchsin
激发波长
(nm)
336
372
385
504
492
512/598
542
492
370-415
370-415
578
567
548
542
518
518
520
351
546
360
发射波长
(nm)
490
480
502
529
518
备注
563/668
Ratio Dye, pH
568
518
520-540
520-540
604
591
552
568
543
543
548
430
647
449, 455
412, 430
471,
474
344
540
445
630
ACMA
(9-Amino-6-chloro-2-methoxyacridine)
412, 430
471, 474
Acridine Orange + DNA
Acridine Orange + RNA
Acridine Orange, both DNA & RNA
Acridine Red
Acridine Yellow
Acriflavin
AcriflavinFeulgen
SITSA
Aequorin (Photoprotein)
AFPs - AutoFluorescent Protein -
(Quantum
Biotechnologies) see sgGFP,
sgBFP
Alexa Fluor 350?
Alexa Fluor 430?
Alexa Fluor 488?
Alexa Fluor 532?
502
460
440-480
455-600
470
436
355-425
346
342
431
526
650
520-650
560-680
550
520
460
466
442
441
540
Photoprotein
495, 492
519, 520
531,
532
553, 554
Alexa Fluor 546?
Alexa Fluor 568?
Alexa Fluor 594?
Alexa Fluor 633?
Alexa Fluor 647?
Alexa Fluor 660?
Alexa Fluor 680?
Alizarin Complexon
Alizarin
Red
Allophycocyanin (APC)
AMC, AMCA-S
AMCA
(Aminomethylcoumarin)
AMCA-X
Aminoactinomycin D
Aminocoumarin
Aminomethylcoumarin (AMCA)
Anilin Blue
Anthrocyl
stearate
APC (Allophycocyanin)
APC-Cy7
APTRA-BTC = Ratio
Dye, Zn2+
APTS
Astrazon
Brilliant Red 4G
Astrazon Orange R
Astrazon Red 6B
Astrazon
Yellow 7 GLL
Atabrine
ATTO-
TAG? CBQCA
ATTO-
TAG? FQ
Auramine
Aurophosphine G
Aurophosphine
BAO 9
(Bisaminophenyloxadiazole)
BCECF (high
pH)
BCECF (low pH)
BerberineSulphate
Beta
Lactamase
556, 557
572, 573
577, 578
603
590, 594
617,
618
632
647
668
679
580
530-560
650
666
698
702
624-645
580
530-560,
580,
630, 645
655, 660
345
345
347
353
555
346
350
345
347
360-381
445
425
444
442
655
442
445
425
444
600
446
630, 645
655, 660
625-650
466/380
424
500
470
520
450
436
465
486
460
450
450-490
365
755
520/530
Ratio Dye, Zn2+
505
585
540
595
480
490
560
591
550
580
515
395
492, 503
520,528
482
430
409
520
550
447, 520
blue shifted GFP
BFP blue
shifted GFP (Y66H)
Blue Fluorescent
Protein
BFP/GFP FRET
Bimane
Bisbenzamide
Bisbenzimide
(Hoechst)
bis-BTC = Ratio Dye, Zn2+
Blancophor FFG
Blancophor SV
BOBO?
-1
BOBO?
-3
Bodipy 492/515
Bodipy 493/503
Bodipy
500/510
Bodipy 505/515
Bodipy 530/550
Bodipy
542/563
Bodipy 558/568
Bodipy 564/570
Bodipy
576/589
Bodipy 581/591
Bodipy 630/650-X
Bodipy
650/665-X
Bodipy 665/676
BodipyFl
Bodipy FL ATP
BodipyFl-Ceramide
Bodipy R6G
SE
Bodipy TMR
Bodipy TMR-X
conjugate
Bodipy TMR-X, SE
Bodipy TR
Bodipy TR ATP
Bodipy TR-X SE
BO-
PRO?
-1
BO-
PRO?
-3
Brilliant Sulphoflavin FF
BTC - Ratio Dye Ca2+
381,
382,
445, 447,
(Y66H)
383
398
360
360
455/405
390
370
462
570
490
533
509
502
528
543
558
564
579
584
625
647
605
448
490
461
461
Blue Fluorescent
Protein
529/505
Ratio Dye, Zn2+
470
435
481
602
515
549
515
510
547
563
569
570
590
592
642
665
676
504, 505
511, 513
505
505
528
542
544
544
589
591
588
462
544
430
464/401
514
511
547
574
573
570
617
620
616
481
570
520
533/529
Ratio Dye Ca2+
BTC-5N - atio Dye, Zn2+
Calcein
Calcein Blue
Calcium
Crimson?
Calcium Green
Calcium
Green-1 Ca2+ Dye
Calcium Green-2 Ca2+
Calcium Green-5N Ca2+
Calcium Green-C18 Ca2+
Calcium Orange
Calcofluor
White
Carboxy-X-rhodamine (5-ROX)
Cascade Blue?
459/417
494
373
517/532
Ratio Dye, Zn2+
517
440
588, 589
611,
615
501, 506
531
506
506/503
506
509
549
531
536
532
530
575
576
445
601
420
423
550
552
470
520
474, 475,
Ca2+
Dye
Ca2+
Ca2+
Ca2+
385, 395,
437, 440,
405
576
377
398
399
399
400
410
494
430, 433,
Cascade Yellow
Catecholamine
CCF2 (GeneBlazer)
CFDA
CFP - Cyan Fluorescent Protein
CFP/YFP FRET
Chlorophyll
Chromomycin A
Chromomycin A
CL-NERF (Ratio Dye, pH)
CMFDA
Coelenterazine Ca2+
Dye, bioluminescence
Cyan
Fluorescent
Protein
Ratio Dye, pH
Ca2+ Dye,
bioluminescence,
native molecule
Ca2+ Dye,
bioluminescence
Ca2+ Dye,
bioluminescence
Ca2+ Dye,
bioluminescence
Ca2+ Dye,
476,
436, (453)
(501)
480
436-460
445
504/514
494
(429)
650
470
575
540
520
465
Coelenterazinecp (Ca2+
Dye,)
Coelenterazine f
Coelenterazinefcp
Coelenterazine h
(430)
(437)
(437)
442
473
452
464
bioluminescence
Coelenterazinehcp
Coelenterazineip
Coelenterazine n
Coelenterazine O
CoumarinPhalloidin
C-phycocyanine
CPM
Methylcoumarin
CTC
CTC
Formazan
Cy2?
Cy3.1 8
Cy3.5?
Cy3?
Cy5.1 8
Cy5.5?
Cy5?
Cy7?
Cyan GFP
cyclic AMP Fluorosensor (FiCRhR)
CyQuant Cell Proliferation Assay
Dabcyl
Dansyl
Dansyl Amine
DansylCadaverine
Dansyl
Chloride
Dansyl DHPE
Dansyl
fluoride
DAPI
Dapoxyl
Dapoxyl 2
Dapoxyl 3
DCFDA
DCFH
(DichlorodihydrofluoresceinDiacetate)
(433)
(431)
460
387
384
400-450
489
554
581
514
552
554
649
675
649
444
441
467
575
470
469
602
506
568
598
566
570
666
695
666
670
Ca2+ Dye,
bioluminescence
Ca2+ Dye,
bioluminescence
Ca2+ Dye,
bioluminescence
Methylcoumarin
710, 743
767, 805
433 (453)
475
(501)
500
480
453
340
337
335
372
336
356
359
403
374
373
504
505
517
520
578
517
518
518
517
none
461
580
574
574
529
535
Cell
Proliferation
Assay
DDAO
DHR (Dihydorhodamine
123)
Di-4-ANEPPS
Di-8-ANEPPS
(non-ratio)
DiA (4-Di-16-ASP)
DichlorodihydrofluoresceinDiacetate
(DCFH)
DiD - Lipophilic Tracer
DiD (DiIC18(5))
DIDS
Dihydorhodamine 123 (DHR)
DiI (DiIC18(3))
Dinitrophenol
DiO
(DiOC18(3))
DiR
DiR
(DiIC18(7))
DM-NERF (high pH)
DNP
Dopamine
DsRed
DTAF
DY-630-NHS
463
505
496
488
498
456
505
644
644
341
505
607
534
705
605
713
591
535
665
665
415
535
Lipophilic Tracer
549, 551
565
349
484, 487
501,
502
748
750
497/510
349
340
558
494
621
780
779
540
Lipophilic Tracer
Ratio Dye, pH
490-520
583
520
660
Red
fluorescent
protein
Hemicyane
label for proteins
and
DNA
Hemicyane
DY-635-NHS
634
664
label for proteins and
DNA
Enhanced Blue
Fluorescent Protein
Enhanced
Cyan
Fluorescent Protein
Enhanced Green
Fluorescent
Protein
EBFP
ECFP
EGFP
ELF 97
Eosin
Erythrosin
Erythrosin ITC
Ethidium
Bromide
Ethidiumhomodimer -1 (EthD-1)
Euchrysin
383
436
447
474
488, 498
507, 516
345
524
530
545
529, 532
554, 555
529
555
510, 523
595, 605
528
430
617
540
EukoLight
Europium (III) chloride
EYFP
Fast Blue
FDA
Feulgen (Pararosaniline)
FIF
(Formaldehyd Induced Fluorescence)
FITC
FITC Antibody
Flazo Orange
Fluo-3
Fluo-4
Fluorescein (FITC)
Fluorescein Diacetate
Fluoro-Emerald
Fluoro-Gold
(Hydroxystilbamidine)
Fluor-Ruby
FluorX
FM
1-
43?
FM 4-46
Fura Red? (high pH)
Fura Red?/Fluo
-3
Fura-2, high calcium
Fura-2,
low calcium
Fura-2/BCECF
Genacryl Brilliant Red B
Genacryl Brilliant Yellow 10GF
Genacryl Pink 3G
Genacryl
Yellow 5GF
GeneBlazer (CCF2)
GFP (S65T)
GFP red shifted
(rsGFP)
GFP wild type, non-UV
excitation (wtGFP)
GFP wild type, UV
excitation (wtGFP)
GFPuv
Gloxalic Acid
Granular Blue
Haematoporphyrin
Enhanced Yellow
Fluorescent Protein
513, 520
527, 532
360
494
570
405
440
520
625
433
490, 494
520, 525
493
375-530
517
612
480
–
506,
520, 527
506
494
516
490, 494
520, 525
494
495
361
555
494
479
515
572
335
363
520
430
470
430
498
498
475
395
385
405
355
530-560
520
524
536
582
520
598
640
657
505
512
590
485
583
475
516
516
509
509
508
460
425
580
Excitation ratio dye
Excitation ratio dye