-
荧光素名称
1,5IAEDANS
1,8-ANS
4-Methylumbelliferone
5-carb
oxy-2,7-
dichlorofluorescein
5-Carboxyfluorescein(5-FAM)
激发波长
(nm)
336
372
385
504
492
发射波长
(nm)
490
480
502
529
518
563/668
568
518
520-540
520-540
604
591
552
568
543
543
548
430
647
备注
RatioDye,pH
5-
512/598
Carboxynapthofluorescein(pH10)
< br>5-
Carboxytetramethylrhodamine(5-
542
TAMRA)
5-FAM(5-Carboxyfluorescein)
5-HAT(HydroxyTryptamine)
5-HydroxyTryptamine(HAT)
5-ROX(carboxy-X-rhodamine)
492
370-415
370-415
578
567
5-TAMRA(5-
548
Carboxytetramethylrhodamine)
542
6-Carboxyrhodamine6G
6-CR6G
6-JOE
7-Amino-4-methylcoumarin
518
518
520
351
7-AminoactinomycinD(7-AAD)
546
7-Hydroxy-4-methylcoumarin
9
-Amino-6-chloro-2-
methoxyacridine
ABQ
AcidFuchsin
A
CMA(9-Amino-6-chloro-2-
methoxyacridine)
AcridineOrange+DNA
AcridineOrange+RNA
360
412,430
344
540
412,430
502
460
449,455
471,474
445
630
471,474
526
650
520-650
560-680
550
520
460
466
Photoprotein
AcridineOrange,bothDNA&RNA 440-480
AcridineRed
AcridineYellow
Acriflavin
AcriflavinFeulgenSITSA
Aequorin(Photoprotein)
AFPs-
AutoFluorescentProtein-
(QuantumBiotechn
ologies)seesg
GFP,sgBFP
AlexaFluor350
AlexaFluor430
AlexaFluor488
AlexaFluor532
455-600
470
436
355-425
346
342
431
495,492
531,532
442
441
540
519,520
553,554
AlexaFluor546
AlexaFluor568
AlexaFluor594
AlexaFluor633
AlexaFluor647
AlexaFluor660
AlexaFluor680
AlizarinComplexon
AlizarinRed
Allophycocyanin(APC)
AMC,AMCA-S
AMCA(Aminomethylcoumarin)
AMCA-X
AminoactinomycinD
Aminocoumarin
Aminomethylcoumarin(AMCA)
AnilinBlue
Anthrocylstearate
APC(Allophycocyanin)
556,557
577,578
590,594
632
647
668
679
572,573
603
617,618
650
666
698
702
530-560,580
580,624-645
530-560
630,645
345
345
347
353
555
346
350
345
347
580
655,660
445
425
444
442
655
442
445
425
444
600
446
655,660
360-381
630,645
APC-Cy7
APTRA-BTC=RatioDye,Zn2+
APTS
AstrazonBrilliantRed4G
AstrazonOrangeR
AstrazonRed6B
AstrazonYellow7GLL
Atabrine
ATTO-TAGCBQCA
ATTO-TAGFQ
Auramine
AurophosphineG
Aurophosphine
625-650
466/380
424
500
470
520
450
436
465
486
460
450
450-490
755
520/530
505
585
540
595
480
490
560
591
550
580
515
395
520,528
520
550
447,520
RatioDye,Zn2+
blueshiftedGFP(Y66H)
BlueFluorescentProtein
BAO9(
Bisaminophenyloxadiazol
365
e)
BCECF(highpH)
BCECF(lowpH)
BerberineSulphate
BetaLactamase
BFPblueshiftedGFP(Y66H)
BlueFluorescentProtein
492,503
482
430
409
381,382,383
445,447,448
BFP/GFPFRET
Bimane
Bisbenzamide
Bisbenzimide(Hoechst)
bis-
BTC=RatioDye,Zn2+
BlancophorFFG
BlancophorSV
BOBO-1
BOBO-3
Bodipy492/515
Bodipy493/503
Bodipy500/510
Bodipy505/515
Bodipy530/550
Bodipy542/563
Bodipy558/568
Bodipy564/570
Bodipy576/589
Bodipy581/591
398
360
360
455/405
390
370
462
570
490
533
509
502
528
543
558
564
579
584
490
461
461
529/505
470
435
481
602
515
549
515
510
547
563
569
570
590
592
RatioDye,Zn2+
Bodipy630/650-X
Bodipy650/665-X
Bodipy665/676
BodipyFl
Bodipy FLATP
BodipyFl-
Ceramide
BodipyR6GSE
BodipyTMR
BodipyTMR-
Xconjugate
BodipyTMR-X,SE
BodipyTR
BodipyTRATP
BodipyTR-XSE
BO-PRO-1
BO-PRO-3
BrilliantSulphoflavinFF
BTC-
RatioDyeCa2+
BTC-5N-atioDye,Zn2+
Calcein
CalceinBlue
625
647
605
504,505
505
505
528
542
544
544
589
591
588
462
544
430
464/401
459/417
494
373
642
665
676
511,513
514
511
547
574
573
570
617
620
616
481
570
520
533/529
517/532
517
440
RatioDyeCa2+
RatioDye,Zn2+
CalciumCrimson
CalciumGreen
CalciumGreen-1Ca2+Dye
CalciumGreen-2Ca2+
CalciumGreen-5NCa2+
CalciumGreen-C18Ca2+
CalciumOrange
CalcofluorWhite
588,589
501,506
506
506/503
506
509
549
611,615
531
531
536
532
530
575
576
Ca2+Dye
Ca2+
Ca2+
Ca2+
385,395,405
437,440,445
601
420
423
550
552
470
Carboxy-X-rhodamine(5-ROX)
576
CascadeBlue
CascadeYellow
Catecholamine
CCF2(GeneBlazer)
CFDA
CFP-CyanFluorescentProtein
CFP/YFPFRET
Chlorophyll
ChromomycinA
ChromomycinA
CL-NERF(RatioDye,pH)
CMFDA
377
398
399
399
400
410
494
520
430,433,436,
4
74,475,476,
CyanFluorescentProtein
(453)
(501)
480
436-460
445
504/514
494
650
470
575
540
520
465
RatioDye,pH
Ca2+
Dye,bioluminescence
CoelenterazineCa2+Dy
e,biolumi
(429)
nescence
Coelenterazinecp(Ca2+Dye,)
Coelenterazinef
Coelenterazinefcp
Coelenterazineh
Coelenterazinehcp
Coelenterazineip
Coelenterazinen
CoelenterazineO
CoumarinPhalloidin
C-phycocyanine
CPMMethylcoumarin
CTC
CTCFormazan
Cy2
Cy3
Cy5
Cy7
(430)
(437)
442
473
452
464
444
441
467
575
470
,
nativemolecule
Ca2+Dye,bioluminescence
Ca2+Dye,bioluminescence
Ca2+Dye,bioluminescence
Ca2+Dye,bioluminescence
Ca2+Dye,bioluminescence
Ca2+Dye,bioluminescence
Ca2+Dye,bioluminescence
(437)
(433)
(431)
460
387
Methylcoumarin
384
400-450
469
602
489
554
581
514
552
554
649
675
649
710,743
506
568
598
566
570
666
695
666
670
767,805
CyanGFP
433(453)
475(501)
517
520
CellProliferationAssay
cycli
cAMPFluorosensor(FiCRhR
500
)
CyQuantCellProliferationAssay
480
Dabcyl
Dansyl
DansylAmine
DansylCadaverine
DansylChloride
DansylDHPE
Dansylfluoride
DAPI
Dapoxyl
Dapoxyl2
Dapoxyl3
DCFDA
453
340
337
335
372
336
356
359
403
374
373
504
578
517
518
518
517
none
461
580
574
574
529
535
607
534
705
DCFH(Dichlorodihydrofluores
cei
505
nDiacetate)
DDAO
DHR(Dihydorhodamine123)
Di-4-ANEPPS
463
505
496
Di-8-ANEPPS(non-ratio)
DiA(4-Di-16-ASP)
488
498
456
605
713
591
535
665
665
415
535
565
LipophilicTracer
Dichlorodih
ydrofluoresceinDiace
505
tate(DCFH)
DiD-
LipophilicTracer
644
DiD(DiIC18(5))
DIDS
Dihydorhodamine123(DHR)
DiI(DiIC18(3))
Dinitrophenol
DiO(DiOC18(3))
DiR
DiR(DiIC18(7))
DM-
NERF(highpH)
DNP
Dopamine
DsRed
DTAF
DY-630-NHS
DY-635-NHS
EBFP
ECFP
EGFP
644
341
505
549,551
349
484,487
748
750
497/510
349
340
558
494
621
634
383
436
488,498
LipophilicTracer
501,502
780
779
540
RatioDye,pH
490-520
583
520
660
664
447
474
507,516
Redfluorescentprotein
Hemicyane
labelforproteinsandDNA
Hemicyane
labelforproteinsandDNA
Enhan
cedBlueFluorescent
Protein
En
hancedCyanFluorescent
Protein
EnhancedGreenFluorescen
tProtein
ELF97
Eosin
Erythrosin
ErythrosinITC
EthidiumBromide
345
524
529,532
529
510,523
530
545
554,555
555
595,605
617
540
EnhancedYellowFluoresce
ntProtein
Ethidiumhomodimer-1(EthD-1)
528
Euchrysin
EukoLight
Europium(III)chloride
EYFP
FastBlue
FDA
Feulgen(Pararosaniline)
430
513,520
360
494
570
527,532
440
520
625
433
520,525
517
612
FIF(FormaldehydInducedFluor
es
405
cence)
FITC
FITCAntibody
FlazoOrange
Fluo-3
490,494
493
375-530
480
–
506,506
520,527
Fluo-4
Fluorescein(FITC)
FluoresceinDiacetate
Fluoro-
Emerald
Fluoro-
Gold(Hydroxys
tilbamidine)
Fluor-Ruby
FluorX
FM1-43
FM4-46
FuraRed(highpH)
FuraRed/Fluo-3
Fura-2,highcalcium
Fura-2,lowcalcium
Fura-2/BCECF
GenacrylBrilliantRedB
GenacrylBrilliantYellow10GF
GenacrylPink3G
GenacrylYellow5GF
GeneBlazer(CCF2)
GFP(S65T)
494
490,494
494
495
361
555
494
479
515
572
516
520,525
520
524
536
582
520
598
640
657
Excitationratiodye
Excitationratiodye
335
363
505
512
520
430
470
430
590
485
583
475
498
516