以STO细胞和hELF作为胚胎生殖细胞培养饲养层的比较

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以

STO

细胞和

hELF

作为胚胎生殖细胞培养饲

养层的比较

作者：刘永刚，李芳菲，陈地龙，王莎莉，王亚平

【关键词】

STO

细胞；胚胎肺成纤维细胞；胚胎生殖细胞；人类

Comparison between STO cells and hELF used as feeder

layers of embryonic germ cells

【

Abs tract

】

AIM:

To

compare

the

influence

on

the

growth

of human embryonic germ (EG) cells by SIM mouse embryoderived

thioguanine

and

ouabain

resistant

(STO)

cells

and

human

embryonic

lung

fibroblasts

（

hELF

）

respectively

as

feeder

layers

and

the

changes

of

the

two

kinds

of

the

feeder

layers

themselves

processed with mitomycin C. METHODS: We isolated and cultured

hELF of 3-4 month embryos. The changes of the two kinds of the

feeder

layers

themselves

treated

with

mitomycin

C

were

observed.

STO

and

hELFs

erved

as

feeder

layer

to

cultivate

human

EG

formation rate of embryonic germ cell colony and the

expression

of

EG

cell

surface

markers(SSEA1,

SSEA4)

expression

were

detected.

to

discuss

the

influence

on

human

EG

cell

growth.

RESULTS: HELF treated by mitomycin C survived longer than STO

cells

treated

by

mitomycin

C

did.

There

was

no

obvious

difference

between

the

two

kinds

of

feeder

layers

in

the

formation

rate

and

immunologic

markers

(SSEA1,

SSEA4)

of

EG

cell

colony.

CONCLUSION:

1

页

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HELF as feeder layers is better than STO cells in culturing EG

cells.

【

Key words

】

STO cells; embryonic lung fibroblasts;

embryonic germ cells; humans

【摘要】

目的：

比较以

STO

细胞和人胚胎肺成纤维细胞

(hELF)

作为饲养层对人胚胎生殖嵴细胞

(EG)

生长的影响 以及经丝裂酶素

C

处

理后的两种饲养层 的变化

.

方法：

分离、培养

3~4

mo

，观察经

丝裂酶素

C

处理

STO

细胞和

hELF

后形态学变化，以

STO

细胞和

hELF

作为饲养层培养人

EG

细胞，计数

EG

细胞集落的形成率 以及免疫组化

检测

EG

细胞表面标志物 抗阶段特异性胚胎抗原

1(SSEA1)

和抗阶段特