-
20: AF234299 Binary vector
pCAMBIA-1303, complete sequence PubMed, Protein,
Related
Sequences, Taxonomy
LOCUS AF234299
12361 bp DNA circular SYN 24-APR-2000
DEFINITION Binary vector pCAMBIA-1303,
complete sequence.
ACCESSION AF234299
VERSION AF234299.1 GI:7638078
KEYWORDS .
SOURCE
Binary vector pCAMBIA-1303.
12361
ecor1= 11548
nhe1 2540
5981
ORGANISM Binary
vector pCAMBIA-1303
artificial sequence; vectors.
REFERENCE
1 (sites)
AUTHORS Hajdukiewicz,P.,
Svab,Z. and Maliga,P.
TITLE The
small, versatile pPZP family of Agrobacterium
binary vectors
for plant
transformation
JOURNAL Plant Mol.
Biol. 25 (6), 989-994 (1994)
MEDLINE
95002787
PUBMED 7919218
REFERENCE 2 (bases 1 to 12361)
AUTHORS Roberts,C., Rajagopal,S.,
Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K.,
Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I.,
Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and
Jefferson,R.A.
TITLE A
comprehensive set of modular vectors for advanced
manipulations
and efficient
transformation of plants
JOURNAL
Unpublished
REMARK Full
description of constructs
REFERENCE 3
(bases 1 to 12361)
AUTHORS
Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A.,
Yang,W.,
Nugrohu,S.,
Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N.,
Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and
Jefferson,R.A.
TITLE Direct
Submission
JOURNAL Submitted
(15-FEB-2000) CAMBIA, Clunies Ross St, Black
Mountain /
GPO Box 3200,
Canberra, ACT 2601, Australia
FEATURES
Location/Qualifiers
source
1..12361
/organism=
/db_xref=
GUS GFP 2.(1807).2569
/note=
/codon_start=1
/produ11ct=
/protein_id=
/db_xref=
/translation=
QESRA
IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
GKV
WVNNQEVMEHQGGY
TPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
KKQSYFHDFFNYAGIHRS
VMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVS
VELRDADQQVVATGQGTS
GTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGI
RSVAVKGQQFLINHKPFY
FTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTS
HYPYAEEMLDWADEHGIV
VIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQA
HLQAIKELIARDKNHPSV
VMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVN
VMFCDAHTDTISDLFDVL
CLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIIT
EYGVDTLAGLHSMYTDMW
SEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGIL
RVGGNKKGIFTRDRKPKS
AAFLLQKRWTGMNFGEKPQQGGKQASKGEELFTGVVPILV
ELDGDVNGHKFSVSGEGE
GDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRY
PDHMKRHDFFKSAMPEGY
VQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKE
DGNILGHKLEYNYNSHNV
YIMADKQKNGIKANFKTRHNIEDGGVQLADHYQQNTPIGD
GPVLLPDNHYLSTQSALS
KDPNEKRDHMVLLEFVTAAGITHGMDELYKASHHHHHHV
misc_feature 1088..1090
/note=
misc_feature 2598..2850
/note=
misc_feature 2888..2913
/note=
misc_feature complement(3954..4954)
/note=
rep_origin
complement(5547..6547)
/note=
misc_feature
complement(6957..7217)
/note=
rep_origin
complement(7357..7637)
/note=
CDS
complement(7928..8722)
/note=
pIG121Hm
/codon_start=1
/product=
/protein_id=
/db_xref=
/translation=
MTDSR
YKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCS
EEY
EDEQSPEKIIELYA
ECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
EDTPFKDPRELYDFLKTE
KPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
YDIAFCVRSIREDIGEEQ
YVELFFDLLGIKPDWEKIKYYILLDELF
misc_feature 9147..9172
/note=
misc_feature 9239..9447
/note=
CDS complement(9463..10488)
/note=
/codon_start=1
/product=
/protein_id=
/db_xref=
/translation=
GYVLR
VNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVT
LQD
LPETELPAVLQPVA
EAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQAL
DELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWR
PWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCD
AIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKK
promoter complement(10524..11331)
/note=
promoter 11452..11506
/note=
CDS 11534..11767
/codon_start=1
/product=
/protein_id=
/db_xref=
/translation=
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC
misc_feature 11576..11626
/note=
promoter 11818..12355
/note=
BASE COUNT 3041 a 3276 c
3276 g 2768 t
ORIGIN Bgl
II Spe I
1 catggtagat
ctgactagtt tacgtcctgt agaaacccca acccgtgaaa
tcaaaaaact
61 cgacggcctg
tgggcat
i
tca gtctggatcg
cgaaaactgt ggaattgatc agcgttggtg
121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca
ggcagtttta acgatcagtt
181
cgccgatgca gatattcgta attatgcggg caacgtctgg
tatcagcgcg aagtctttat
241
accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc
gatgcggtca ctcattacgg
301
caaagtgtgg gtcaataatc aggaagtgat ggagcatcag
ggcggctata cgccatttga
361
agccgatgtc acgccgtatg ttattgccgg gaaaagtgta
cgtatcaccg tttgtgtgaa
421
caacgaactg aactggcaga ctatcccgcc gggaatggtg
attaccgacg aaaacggcaa
481
gaaaaagcag tcttacttcc atgatttctt taactatgcc
ggaatccatc gcagcgtaat
541
gctctacacc acgccgaaca cctgggtgga cgatatcacc
gtggtgacgc atgtcgcgca
601
agactgtaac cacgcgtctg ttgactggca ggtggtggcc
aatggtgatg tcagcgttga
661
actgcgtgat gcggatcaac aggtggttgc aactggacaa
ggcactagcg ggactttgca
721
agtggtgaat ccgcacctct ggcaaccggg tgaaggttat
ctctatgaac tgtgcgtcac
781
agccaaaagc cagacagagt gtgatatcta cccgcttcgc
gtcggcatcc ggtcagtggc
841
agtgaagggc caacagttcc tgattaacca caaaccgttc
tactttactg gctttggtcg
901
tcatgaagat gcggacttac gtggcaaagg attcgataac
gtgctgatgg tgcacgacca
961
cgcattaatg gactggattg gggccaactc ctaccgtacc
tcgcattacc cttacgctga
1021
agagatgctc gactgggcag atgaacatgg catcgtggtg
attgatgaaa ctgctgctgt
1081
cggctttcag ctgtctttag gcattggttt cgaagcgggc
aacaagccga aagaactgta
1141
cagcgaagag gcagtcaacg gggaaactca gcaagcgcac
ttacaggcga ttaaagagct