同尾酶汇总表

连接互相匹配的粘性末端，产生新的酶切位点

通常连接互相匹配的粘性末端可以产生新的酶切位点。以下组合是通过计算机程序计算出来的，尽管我们希望能尽 量保证其准确性，

但有些未经实验证实，因此不能保证

100%

的可行性。如果有同裂酶存在，则只列出其中的一个酶。本表中列出的内切酶均为已商品 化

的内切酶。

识别序列不够严谨的内 切酶（例如，识别多个序列的酶）只在括号内标出相关的序列，但注意这些酶不只切割该序列。

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表示连接产物不能被再切割。

酶

Acc65 I

(G/GTACC)

Acc I

(GT/CGAC)

(GT/CGAC)

Aci I

(C/CGC)

Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I

Cla I, BstB I, Taq I

Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I

BsaH I (GR/CGCC), HinP1 I, Nar I

Hpa II

Acl I

(AA/CGTT)

Afl III

Acc I (GT/CGAC), Aci I, Cla I, BstB I, HinP1 I, Hpa II, Nar I, Taq I

--

Taq I

--

Aci I

Hpa II

--

连接到

Ban I (G/GTACC)

BsiW I, BsrG I

可进行切割的酶

Acc65 I, Ban I, Kpn I, Nla IV, Rsa I

Rsa I

(A/CGCGT)

(A/CATGT)

(A/CGCGT)

Age I

(A/CCGGT)

Asc I, BssH II

BspH I, Nco I, Pci I

Mlu I

Ava I (C/CCGGG), Xma I

BsaW I, BspE I

BsrF I (A/CCGGT), SgrA I (CA/CCGGTG)

NgoM IV

BstU I, Hha I

Nla III

Afl III, BstU I, Mlu I

Hpa II, Nci I, ScrF I

BsaW I, Hpa II

Age I, BsaW I, BsrF I, Hpa II

BsrF I, Hpa II

Apa I, Ban II, Bsp120 I, Bsp1286 I, Hae III, Nla IV,

Sau96 I

Bsg I

Apa I

(GGGCC/C)

ApaL I

(G/TGCAC)

Apo I

(A/AATTY)

(G/AATTY)

(R/AATTY)

Asc I

(GG/CGCGCC)

Ase I

(AT/TAAT)

AsiS I

(GCGAT/CGC)

Ban II (GGGCC/C), Bsp1286 I (GGGCC/C)

Sfc I (C/TGCAG)

EcoR I

EcoR I

Mfe I, Tsp509 I

Afl III (A/CGCGT), Mlu I

BssH II

Bfa I, Csp6 I, Nde I

Mse I

BsiE I (CGAT/CG)

Pac I

Apo I, Tsp509 I

Apo I, EcoR I, Tsp509 I

Tsp509 I

BstU I, Hha I

BssH II, BstU I, Cac8 I, Hha I

--

Mse I

Dpn II, Pvu I

Mse I

Pvu I

Ava I

(C/CCGGG)

(C/TCGAG)

(C/TCGAG)

(C/CCGGG)

Ava II

(G/GWCC)

Age I, BsaW I, BspE I, BsrF I (R/CCGGY), NgoM IV, SgrA I

(CR/CCGGYG)

Xho I

Sal I

Xma I

PpuM I (RG/GACCY)

Rsr II

PpuM I (RG/GTCCY)

Avr II

(C/CTAGG)

BamH I

(G/GATCC)

Nhe I, Spe I, Xba I

Sty I (C/CTAGG)

Bcl I, Dpn II

Bgl II, BstY I (R/GATCY)

BstY I (G/GATCC)

Ban I

(G/GTACC)

(G/GCGCC)

(G/GTACC)

Ban II

Acc65 I

Kas I

BsiW I, BsrG I

Dpn II, Pvu I

Hpa II, Nci I, ScrF I

Ava I, Taq I, Xho I

Taq I

Ava I, BsaJ I, Hpa II, Nci I, ScrF I, Sma I

Ava II, Nla IV, Sau96 I

Ava II, Sau96 I

Ava II, BsmF I, Nla IV, Sau96 I

Bfa I

Avr II, Bfa I, BsaJ I, Sty I

Alw I, Dpn II

Alw I, BstY I, Dpn II

Alw I, BamH I, BstY I, Dpn II, Nla IV

Acc65 I, Ban I, Kpn I, Nla IV, Rsa I

Ban I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IV

Rsa I

(GGGCC/C)

(GAGCT/C)

Bcl I

(T/GATCA)

Bfa I

(C/TAG)

Bgl II

(A/GATCT)

BsaH I

(GR/CGYC)

(GA/CGYC)

(GG/CGYC)

(GG/CGYC)

(GA/CGYC)

(GG/CGYC)

BsaW I

(W/CCGGW)

Apa I, Bsp1286 I (GGGCC/C)

Bsp1286 I (GAGCT/C), Sac I

BamH I, BstY I (R/GATCY)

Bgl II, Mbo II

Ase I, Csp6 I, Mse I, Nde I

BamH I, BstY I (R/GATCY)

Bcl I, Dpn II

Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I

Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I

Alw I, Dpn II

Dpn II

--

Alw I, BstY I, Dpn II

Dpn II

Acc I (GT/CGAC), Cla I, BstB I, Taq I

Aci I, HinP1 I

Aci I, HinP1 I

Hpa II

Nar I

Nar I

Age I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)

Ava I (C/CCGGG), Xma I

BspE I

BsrF I (R/CCGGY), NgoM IV

NgoM IV

--

Hga I

Hha I

Aci I

BsaH I, Hga I

Ban I, BsaH I, Hae II, Hha I, Nar I, Nla IV

Age I, BsaW I, BsrF I, Hpa II

Hpa II, Nci I, ScrF I

BsaW I, BspE I, Hpa II

BsrF I, Hpa II

Hpa II

BsiE I

(CGAT/CG)

(CGAT/CG)

(CGGC/CG)

BsiHKA I

(GTGCA/C)

Pac I

Pvu I

Sac II

Bsp1286 I (GTGCA/C)

Bsp1286 I (GAGCA/C)

Bsp1286 I (GAGCT/C), Sac I

Nsi I

Pst I, Sbf I

BsiW I

(C/GTACG)

Bsp1286 I

(GGGCC/C)

(GTGCA/C)

(GGGCC/C)

(GAGCT/C)

(GWGCW/C)

(GTGCA/C)

(GTGCA/C)

BspE I

Apa I, Ban II (GGGCC/C)

BsiHKA I

Ban II (GGGCC/C)

Ban II (GAGCT/C), BsiHKA I, Sac I

BsiHKA I

Nsi I

Pst I, Sbf I

Age I, BsaW I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)

Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I

ApaL I, BsiHKA I, Bsp1286 I

Ban II, Bsp1286 I

Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I

BsiHKA I, Bsp1286 I

--

Bsg I

BsaW I, Hpa II

Acc65 I, Ban I (G/GTACC), BsrG I

Mse I

BsiE I, Dpn II, Pvu I

Aci I

BsiHKA I, Bsp1286 I

BsiHKA I, Bsp1286 I

Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac I

--

Bsg I

Rsa I

(T/CCGGA)

Ava I (C/CCGGG), Xma I

BsaW I

BsrF I (R/CCGGY), NgoM IV

Hpa II, Nci I, ScrF I

BsaW I, BspE I, Hpa II

Hpa II

Nla III

BspH I

(T/CATGA)

BsrF I

(A/CCGGY)

(G/CCGGY)

(R/CCGGY)

(A/CCGGY)

(R/CCGGY)

(G/CCGGY)

(CR/CCGGYG)

BsrG I

(T/GTACA)

BssH II

(G/CGCGC)

BstB I

(TT/CGAA)

BstY I

(A/GATCY)

Afl III (A/CATGT), Nco I, Sty I (C/CATGG)

Age I, BsaW I

Age I, BsaW I, NgoM IV

Ava I (C/CCGGG), Xma I

BsaW I, BspE I

BsaW I, BspE I

NgoM IV

SgrA I

Acc65 I, Ban I (G/GTACC), BsiW I

Afl III (A/CGCGT), Mlu I

Asc I

Acc I (GT/CGAC), Cla I, Taq I

Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I

Age I, BsaW I, BsrF I, Hpa II

BsrF I, Hpa II

Hpa II, Nci I, ScrF I

BsaW I, Hpa II

Hpa II

BsrF I, Cac8 I, Hpa II, Nae I

BsrF I, Hpa II

Rsa I

BstU I, Hha I

BssH II, BstU I, Cac8 I, Hha I

Taq I

--

BamH I, Bgl II

Alw I, BstY I, Dpn II