写作指导：如何撰写SCI医学论文之个人体会

b

）由此引申出本研究的目的和研究结果概要；

c

）通过

对上述不足的解决显示本研究的意义所在。下面以我发 表在

国际知名肿瘤学期刊

Oncogene

上的一篇文章的摘要为例：

The histone acetyltransferase TIP60 regulates the

DNA

damage

response

following

genotoxic

stress

by

acetylating histone and remodeling chromatin

（背景

概述）

. However, the molecular mechanisms underlying

the TIP60-dependent response to UV-induced DNA damage

remain poorly understood (

当前 研究的某一不足之处，此

处

用

However

以

示

转

折

).

To

systematically

analyze

proteins that regulate TIP60 activity in response to

UV irradiation

（为解决上述不足提出本研究目的）

, we

performed

a

proteomic

analysis

of

proteins

selectively

bound

to

TIP60

in

response

to

UV

irradiation

using

mass

spectrometry

and

identified

a

novel

regulatory

mechanism by which TIP60 orchestrates transcriptional

activation

of

p53-dependent

checkpoint

response

in

UV-irradiated cells. The initial step of this pathway

involves

UV-induced

association

of

TIP60

with

SUMO-conjugation

enzymes

and

site-specific

sumoylation of TIP60 at lysines 430 and 451 via Ubc9.

This

sumoylation

initiates

the

relocation

of

TIP60

from

nucleoplasm to the promyelocytic leukemia body, which

is essential for the UV-irradiated DNA damage repair

response via a p53-dependent pathway. Significantly,

inhibition of TIP60 sumoylation by overexpression of

non- sumoylatable mutant abrogates the p53-dependent

DNA damage response, demonstrating the importance of

TIP60

sumoylation

in

response

to

UV

irradiation.

Furthermore,

our

biochemical

characterization

demonstrated

that

the

sumoylation

of

TIP60

augments

its

acetyltransferase activity in vitro and in vivo

（研

究手段和主要研究结论的概述。注意这里使用了显示逻辑递

进关系的词）

。

. Thus, this study shed new light on the

function

and

regulation

of

TIP60

activity

in

UV-irradiated DNA damage response