subdivision各种神经行为学评价

;2

分

:< /p>

转动开始后

.

鼠从棒上掉下来

分

:

转动开始前

.

鼠从棒上

掉下来。

10

．

The detection was operated at 1 week after surgery by injecting 0. 5 mg/kg of apomorphine

into

abdominal

cavity

for

observation

of

the

changes

in

The

behaviours

for

once

a

week

and

continuous detected for 6 weeks. If constant right-side rotation occurred and The numbers of The

circles

rotated

were

more

than

210

r

per

30

minutes,

those

rats

were

considered

as

successful

models: if no rotation occurred or The direction of rotation was not constant or constant right-side

or

lell-side

rotation

but

The

speed

was

slower

than

210

r

per

30

minutes,

those

animals

were

considered

as

unsuccessful

models.

In

additional

to

rotation,

other

companioned

abnormal

behaviours also should be observed, such as, tremor, sluggish, grab, and smell exploring, etc.

11. rotarod test

rats

were

placed

on

an

accelerating

rotarod

cylinder,

and

the

time

the

animals

remained

on

the

rotarod was measured. The speed was slowly increased from 4 to 40 rpm within 5 minutes. A trial

ended

if

the

animal

fell

off

the

rungs

or

gripped

the

device

and

spun

around

for

2

consecutive

revolutions

without

attempting

to

walk

on

the

rungs.

The

animals

were

trained

3

days

before

MCAO. The mean duration (in seconds) on the device was recorded with 3 rotarod measurements

1 day before surgery. Motor test data are presented as percentage of mean duration (3 trials) on the

rotarod compared with the internal baseline control (before surgery).

12. adhesive-removal somatosensory test

somatosensory deficit was measured both before and after surgery. All rats were familiarized with

the testing environment. In the initial test, 2 small pieces of adhesive-backed paper dots (of equal

size, 113.1 mm2) were used as bilateral tactile stimuli occupying the distal-radial region on the

wrist of each forelimb. The rat was then returned to its cage. The time to remove each stimulus

from

forelimbs

was

recorded

on

5

trials

per

day.

Individual

trials

were

separated

by

at

least

5

minutes. Before surgery, the animals were trained for 3 days. Once the rats were able to remove

the dots within 10 seconds, they were subjected to MCAO.

13

．

Spontaneous Activity

The animal was observed for 5 minutes in its normal environment (cage). The rat's activity was

assessed by its ability to approach all four walls of the cage. Scores indicate the following: 3, rat

moved

around,

explored

the

environment,

and

approached

at

least

three

walls

of

the

cage;

2,

slightly affected rat moved about in the cage but did not approach all sides and hesitated to move,

although it eventually reached at least one upper rim of the cage; 1, severely affected rat did not

rise up at all and barely moved in the cage; and 0, rat did not move at all.

14

．

Symmetry in the Movement of Four Limbs

The rat was held in the air by the tail to observe symmetry in the movement of the four limbs.

Scores

indicate

the

following:

3,

all

four

limbs

extended

symmetrically;

2,

limbs

on

left

side

extended

less

or

more

slowly

than

those

on

the

right;

1,

limbs

on

left

side

showed

minimal

movement; and 0, forelimb on left side did not move at all.

Julio

H.

Garcia,

MD;

Simone

Wagner,

MD;

Kai-Feng

Liu,

MD;

Xiao-jiang

Hu,

MD,

PhD

。

Neurological

Deficit

and

Extent

of

Neuronal

Necrosis

Attributable

to

Middle

Cerebral

Artery

Occlusion in Rats

。

Stroke. 1995;26:627-635

。