植物表达载体 Pcambia1302 序列图谱

载体质粒图谱和多克隆位点信息

载体简介

载体序列

LOCUS AF234298 10549 bp DNA circular SYN 24-APR-2000

DEFINITION Binary vector pCAMBIA-1302, complete sequence.

ACCESSION AF234298

VERSION AF234298.1 GI:7638073

KEYWORDS .

SOURCE Binary vector pCAMBIA-1302

ORGANISM Binary vector pCAMBIA-1302

other sequences; artificial sequences; vectors.

REFERENCE 1 (sites)

AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.

TITLE The small, versatile pPZP family of Agrobacterium binary vectors

for plant transformation

JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)

PUBMED 7919218

REFERENCE 2 (bases 1 to 10549)

AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,

Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,

Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,

Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.

TITLE A comprehensive set of modular vectors for advanced manipulations

and efficient transformation of plants

JOURNAL Unpublished

REMARK Full description of constructs

REFERENCE 3 (bases 1 to 10549)

AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,

Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,

Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,

Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.

TITLE Direct Submission

JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /

GPO Box 3200, Canberra, ACT 2601, Australia

FEATURES Location/Qualifiers

source 1..10549

/organism=

/mol_type=

/db_xref=

CDS 2..757

/note=

/codon_start=1

/product=

/protein_id=

/db_xref=

/translation=

KLTLK FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQE RTI

FFKDDGNYKTRAEV KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK

QKNGIKANFKTRHNIEDG GVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK

RDHMVLLEFVTAAGITHGMDELYKASHHHHHHV

misc_feature 786..1038

/note=

misc_feature 1076..1101

/note=

misc_feature complement(2142..3142)

/note=

rep_origin complement(3735..4735)

/note=

misc_feature complement(5145..5405)

/note=

rep_origin complement(5545..5825)

/note=

CDS complement(6116..6910)

/note=

pIG121Hm

/codon_start=1

/product=

/protein_id=

/db_xref=

/translation=

MTDSR YKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCS EEY

EDEQSPEKIIELYA ECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE

EDTPFKDPRELYDFLKTE KPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW

YDIAFCVRSIREDIGEEQ YVELFFDLLGIKPDWEKIKYYILLDELF

misc_feature 7335..7360

/note=

misc_feature 7427..7635

/note=

CDS complement(7651..8676)

/note=

/codon_start=1

/product=

/protein_id=

/db_xref=

/translation=

GYVLR VNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVT LQD

LPETELPAVLQPVA EAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY

HWQTVMDDTVSASVAQAL DELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS

EAMFGDSQYEVANIFFWR PWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ

SLVDGNFDDAAWAQGRCD AIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS

TRPRAKK

promoter complement(8712..9492)

/note=

promoter 9640..9694

/note=

CDS 9722..9955

/codon_start=1

/product=

/protein_id=

/db_xref=

/translation=

RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC

misc_feature 9736..9792

/note=

promoter 10006..10543

/note=

ORIGIN

1 catggtagat ctgactagta aaggagaaga acttttcact ggagttgtcc caattcttgt

61 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga

121 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc

181 gtggccaaca cttgtcacta ctttctctta tggtgttcaa tgcttttcaa gatacccaga

241 tcatatgaag cggcacgact tcttcaagag cgccatgcct gagggatacg tgcaggagag

301 gaccatcttc ttcaaggacg acgggaacta caagacacgt gctgaagtca agtttgaggg

361 agacaccctc gtcaacagga tcgagcttaa gggaatcgat ttcaaggagg acggaaacat

421 cctcggccac aagttggaat acaactacaa ctcccacaac gtatacatca tggccgacaa

481 gcaaaagaac ggcatcaaag ccaacttcaa gacccgccac aacatcgaag acggcggcgt

541 gcaactcgct gatcattatc aacaaaatac tccaattggc gatggccctg tccttttacc

601 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga

661 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact

721 atacaaagct agccaccacc accaccacca cgtgtgaatt ggtgaccagc tcgaatttcc

781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg

841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat

901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat

961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat

1021 ctatgttact agatcgggaa ttaaactatc agtgtttgac aggatatatt ggcgggtaaa

1081 cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg aaaaggttta

1141 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact

1201 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct

1261 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt

1321 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac

1381 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc

1441 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc