-
常用荧光团的参数与选择
2015/4/17
Fluorophore
1,8-ANS (BSA)
2-dodecylresorufin (lipid)
5
-(and-6)-carboxy-2',7'-dichlorofluorescein (pH
9.0)
5-carboxytetramethylrhodamine
[5-TAMRA] (MeOH)
5-chloromethylfluorescein (pH 7.2)
5-FAM (pH 9.0)
5-ROX (pH
7.0)
5-TAMRA (pH 7.0)
6-carboxyrhodamine 6G (pH 7.0)
6-JOE (pH 9.0)
7-amino-4-methylcoumarin (pH 7.0)
Peak
Peak
Center
Minimum
Intersection
Optical
Density
Bandwith
Excitation
Emission
Type
Wavelength
Transmission
λ (nm)
OD
(nm)
λ
(nm)
λ (nm)
(nm)
(%)
Excitation
3
77
6
50
85
374.9
477
422.9
Emission
480
3
10
45
Excitation
5
80
3
10
45
581.9
595
589.5
Emission
593
6
40
90
Excitation
5
05
3
10
45
504
524.9
513.8
Emission
520
6
35
90
Excitation
5
43
6
22
90
542.9
567
554.4
Emission
568
3
10
45
Excitation
4
92
3
10
45
490.9
517
501.8
Emission
515
3
10
45
Excitation
4
92
3
10
45
491.9
518.1
503.5
Emission
520
6
35
90
Excitation
5
80
3
10
45
578.2
604
589.7
Emission
600
3
80
60
Excitation
5
50
3
80
60
553
576
563.4
Emission
580
3
10
45
Excitation
5
20
6
35
90
525
547
536
Emission
546
3
10
45
Excitation
5
20
6
35
90
519.8
548
532.7
Emission
550
3
80
60
Excitation
3
40
6
26
75
346
442
396.5
Emission
442
3
10
40
7-aminoactinomycin D (DNA)
7-hydroxy-4-methylcoumarin (pH 9.0)
Acridine orange (DNA)
548.1
360.1
502.1
647.9
447.9
526
441
422.1
540
519
520
517.1
542.9
553
574
572
567.1
601.8
403
513
392.9
409
492.9
505.8
508.8
504.9
529.8
543
563.8
566.9
559.6
Alexa Fluor 350 goat anti-mouse IgG
antibody (pH 8.0)
343
Alexa
Fluor 405 goat anti-mouse IgG antibody (pH 7.2)
401.1
Alexa Fluor 430 goat
anti-mouse IgG antibody (pH 7.2)
431.2
Alexa Fluor 488 dye-labeled
oligonucleotide (H2O)
493.1
Alexa Fluor 488 goat anti-mouse IgG
antibody (pH 8.0)
498.9
Alexa Fluor 488 hydrazide (H2O)
492.8
Alexa Fluor 514 goat
anti-mouse IgG antibody (pH 8.0)
518
Alexa Fluor 532 goat anti-mouse IgG
antibody (pH 7.2)
533.9
Alexa Fluor 546 dye-labeled
oligonucleotide (H2O)
554.1
Alexa Fluor 546 goat anti-mouse IgG
antibody (pH 7.2)
561.3
Alexa Fluor 555 dye-labeled
oligonucleotide (H2O)
552.3
Excitation
5
50
Emission
647
Excitation
3
65
Emission
447
Excitation
5
00
Emission
531
Excitation
3
40
Emission
442
Excitation
4
00
Emission
420
Excitation
4
30
Emission
540
Excitation
4
92
Emission
520
Excitation
5
00
Emission
520
Excitation
4
92
Emission
515
Excitation
5
20
Emission
543
Excitation
5
35
Emission
550
Excitation
5
50
Emission
580
Excitation
5
62
Emission
568
Excitation
5
50
3
3
3
6
6
6
6
3
3
3
3
3
3
6
6
6
3
3
6
6
3
3
3
3
6
3
3
80
10
10
60
24
40
26
10
50
10
10
10
10
35
24
35
10
10
35
22
10
80
80
10
40
10
80
60
45
25
90
90
90
75
40
55
40
40
45
45
90
90
90
45
45
90
90
45
60
60
45
90
45
60
Alexa Fluor 555 goat anti-mouse IgG
antibody (pH 7.2)
553.2
Alexa Fluor 555 hydrazide (H2O)
551.1
567.9
564
603
599.9
614.1
621
618.1
630
627
649.9
647.9
667.9
671
560.2
557.1
590.2
585.8
600.1
605.7
604.2
620.2
589.7
640.2
639.9
659.1
660.6
Alexa Fluor 568 goat anti-mouse IgG
antibody (pH 7.2)
578.9
Alexa Fluor 568 hydrazide (H2O)
Alexa Fluor 594 cadaverine (H2O)
Alexa Fluor 594 dye-labeled
oligonucleotide (H2O)
572.9
587.9
590.9
Alexa
Fluor 594 goat anti-mouse IgG antibody (pH 7.2)
590.2
Alexa Fluor 610 goat
anti-mouse IgG antibody (pH 7.2)
611.9
Alexa Fluor 610-R-phycoerythrin
streptavidin (pH 7.2)
566.9
Alexa Fluor 633 goat anti-mouse IgG
antibody (pH 7.2)
631.1
Alexa Fluor 635 goat anti-mouse IgG
antibody (pH 7.2)
633.1
Alexa Fluor 647 dye-labeled
oligonucleotide (H2O)
650.1
Alexa Fluor 647 goat anti-mouse IgG
antibody (pH 7.2)
649.9
Emission
568
Excitation
5
50
Emission
568
Excitation
5
50
Emission
562
Excitation
5
80
Emission
600
Excitation
5
68
Emission
600
Excitation
5
89
Emission
610
Excitation
5
89
Emission
620
Excitation
5
89
Emission
620
Excitation
6
10
Emission
628
Excitation
5
68
Emission
628
Excitation
6
32
Emission
650
Excitation
6
32
Emission
647
Excitation
6
50
Emission
671
Excitation
6
50
Emission
671
3
3
3
3
6
3
3
3
3
3
3
3
3
3
3
3
6
3
6
4
4
4
3
4
4
4
4
10
80
10
80
40
10
80
10
80
10
10
10
10
10
10
10
40
10
40
10
10
10
10
10
10
10
10
45
60
45
60
90
45
60
45
60
45
45
45
45
45
45
45
90
45
90
85
85
85
45
85
85
85
85
Alexa Fluor
647 hydrazide (H2O)
Alexa Fluor
647-R-phycoerythrin streptavidin (pH 7.2)
649.1
568.2
670
666
690.9
703.9
702
719
776.1
804.9
443.9
660.1
581
483.9
605.1
641
659.2
648.9
679.3
692.8
687.1
708.3
763.6
791.1
394.2
655.6
575.2
472.6
590.2
633.7
Alexa Fluor 660 goat anti-mouse IgG
antibody (pH 7.2)
663.1
Alexa Fluor 680 goat anti-mouse IgG
antibody (pH 7.2)
680.9
Alexa Fluor 680-R-phycoerythrin
streptavidin (pH 7.2)
569.6
Alexa Fluor 700 goat anti-mouse IgG
antibody (pH 7.2)
695.9
Alexa Fluor 750 goat anti-mouse IgG
antibody (pH 7.2)
752.4
Alexa Fluor 790 goat anti-mouse IgG
antibody (pH 7.2)
781.8
Alexa Fluorr 350
Allophycocyanin (pH 7.5)
Amplex UltraRed peroxidation product
(pH 7.5)
BOBO-1 (DNA)
BOBO-3
(DNA)
BODIPY 630-650-X (MeOH)
346.9
651
567.9
461.2
570.2
625.2
Excitation
6
50
Emission
671
Excitation
5
68
Emission
671
Excitation
6
56
Emission
690
Excitation
6
76
Emission
700
Excitation
5
68
Emission
700
Excitation
6
94
Emission
730
Excitation
7
66
Emission
780
Excitation
7
80
Emission
800
Excitation
3
40
Emission
442
Excitation
6
50
Emission
656
Excitation
5
68
Emission
580
Excitation
4
58
Emission
483
Excitation
5
68
Emission
610
Excitation
6
24
4
4
3
4
4
3
3
3
3
3
3
3
3
3
3
3
6
3
4
4
3
3
3
6
3
3
6
10
10
10
10
10
10
10
80
10
80
10
10
10
10
10
10
26
10
10
10
10
10
10
32
10
10
40
85
85
45
85
85
50
50
60
45
60
50
50
50
50
50
50
75
40
85
85
45
45
45
90
45
45
90
BODIPY 650-665-X (MeOH)
BODIPY FL (MeOH)
BODIPY FL
goat anti-mouse IgG antibody (pH 7.2)
BODIPY R6G (MeOH)
BODIPY
TMR-X (MeOH)
645.9
502.1
505.3
528.2
544
664.1
511.1
512
546.9
570.1
573
621
621
514
608.9
529.1
574
674
654.9
506.7
504.1
539.2
559.3
561.4
607.2
608.1
503.6
598.3
517
561.3
637
BODIPY
TMR-X goat anti-mouse IgG antibody (pH 7.2)
544
BODIPY TR-X (MeOH)
BODIPY TR-X phallacidin (pH 7.0)
Calcein (pH 9.0)
Calcium
Crimson (Ca2+)
Calcium Green-1 (Ca2+)
Calcium Orange (Ca2+)
Carboxynaphthofluorescein (pH 10.0)
588
590
493.9
589
507
549.1
598.9
Emission
640
Excitation
6
47
Emission
671
Excitation
5
00
Emission
510
Excitation
5
05
Emission
510
Excitation
5
31
Emission
546
Excitation
5
43
Emission
568
Excitation
5
43
Emission
568
Excitation
5
89
Emission
620
Excitation
5
89
Emission
620
Excitation
4
92
Emission
515
Excitation
5
89
Emission
610
Excitation
5
08
Emission
531
Excitation
5
50
Emission
568
Excitation
6
00
Emission
676
3
3
4
6
6
3
6
6
3
6
3
6
3
3
3
3
3
3
3
3
3
3
6
3
3
3
3
10
10
10
24
84
10
84
40
10
22
10
22
10
10
10
10
10
10
10
10
10
10
40
80
10
80
10
45
45
85
90
90
45
90
90
45
90
45
90
45
45
45
45
45
45
45
45
45
45
90
60
45
60
50
Cascade Blue BSA (pH 7.0)
401.1
419
549.1
675.9
571.9
624.9
452.1
614
525.9
475.1
574.9
565.8
666.1
522.9
519
407.8
472.4
668.6
563.5
613.5
427.7
597.1
470.2
465.3
560.5
559.5
657.9
512.2
505.4
Cascade Yellow goat anti-mouse IgG
antibody (pH 8.0)
399.1
CellMask Deep Red plasma membrane stain
CellMask Orange plasma membrane stain
CellTrace BODIPY TR methyl ester
(lipid)
CellTrace calcein violet (pH
7.2)
CellTracker Red CMTPX
CellTracker Violet BMQC+GSH
CFP (pH 7.4)
CMR (pH 7.4)
Cy3 dye-labeled IgG antibody (pH 7.2)
Cy5 dye-labeled IgG antibody (pH 7.2)
CyQUA GR (DNA)
DAF-FM (NO)
659
556
597.9
400.1
586
404.1
433.1
547
553.9
648.9
502.1
495
Excitation
4
00
Emission
420
Excitation
4
00
Emission
550
Excitation
6
56
Emission
676
Excitation
5
50
Emission
568
Excitation
6
00
Emission
624
Excitation
4
00
Emission
450
Excitation
5
89
Emission
610
Excitation
4
05
Emission
531
Excitation
4
36
Emission
472
Excitation
5
46
Emission
580
Excitation
5
50
Emission
568
Excitation
6
50
Emission
671
Excitation
5
00
Emission
520
Excitation
4
92
3
3
3
3
4
3
3
3
3
6
3
3
3
3
4
6
3
6
3
3
3
3
4
4
6
6
3
50
10
50
80
10
10
80
10
80
40
50
80
10
10
10
40
10
30
10
10
80
10
10
10
24
35
10
55
40
55
60
85
50
60
45
60
90
55
60
45
45
85
90
40
90
45
45
60
45
85
85
90
90
45
DAPI (DNA)
Dapoxyl
(2-aminoethyl)sulfonamide (MeOH)
DDAO
(pH 9.0)
Di-8-ANEPPS (lipid)
DiA (lipid)
DiD (lipid)
DiFMU (pH 3.0)
DiFMU (pH
9.0)
DiI (lipid)
DiO (lipid)
DiR (lipid)
EGFP (pH 7)
ELF 97 alcohol (pH 8.0)
359
373.1
647.7
467
456.9
647.9
319.9
358
550.9
489.2
749.9
488.9
300
460.9
578.9
657.1
631
585.9
669.9
451.1
450
568.9
506
782
508.1
536
401.1
459.2
653.4
536.9
521.5
658.3
370.4
406.5
559.6
497.8
764.7
500.1
405.2
Emission
520
Excitation
3
65
Emission
458
Excitation
3
77
Emission
580
Excitation
6
47
Emission
656
Excitation
4
67
Emission
632
Excitation
4
58
Emission
589
Excitation
6
47
Emission
671
Excitation
3
34
Emission
450
Excitation
3
65
Emission
450
Excitation
5
50
Emission
568
Excitation
4
88
Emission
505
Excitation
7
66
Emission
780
Excitation
4
88
Emission
508
Excitation
3
34
Emission
536
6
3
3
6
3
3
4
3
4
3
3
3
4
3
3
3
3
3
3
3
3
3
3
3
3
3
6
35
10
10
50
10
10
10
10
10
10
10
10
10
10
80
10
80
80
10
10
10
10
10
10
10
10
40
90
25
45
85
45
45
85
45
85
45
45
45
85
25
60
25
60
60
45
45
45
50
50
45
45
25
90
Eosin goat
anti-mouse IgG antibody (pH 8.0)
ER-
Tracker Blue-White DPX (lipid)
Ethidium
bromide (DNA)
Ethidium homodimer-1
(DNA)
FITC goat anti-mouse IgG antibody
(pH 8.0)
FlAsH-CCPGCC
Fluo-3
(Ca2+)
Fluo-4 (Ca2+)
Fluorescein (pH 13.0)
Fluorescein (pH 9.0)
Fluorescein dextran (pH 8.0)
Fluorescein goat anti-mouse IgG
antibody (pH 8.0)
FluoSpheres blue
fluorescent microspheres (H2O)
FluoSpheres crimson fluorescent
microspheres (H20)
525
372.1
300
527
495.1
511
506
494
492.9
489.9
501.1
497.9
355.9
619.9
545.9
556.8
603
616.9
519
530.1
527
515.9
513
513.2
524
517
414
646.1
535.5
422.2
550.2
574.3
506.5
520.1
516.1
504.3
502.1
501.1
511.8
507.1
383.8
635.3
Excitation
5
20
Emission
546
Excitation
3
77
Emission
562
Excitation
3
34
Emission
600
Excitation
5
31
Emission
620
Excitation
4
92
Emission
520
Excitation
5
10
Emission
531
Excitation
5
05
Emission
531
Excitation
4
92
Emission
515
Excitation
4
92
Emission
515
Excitation
4
88
Emission
515
Excitation
5
00
Emission
520
Excitation
5
00
Emission
515
Excitation
3
65
Emission
415
Excitation
6
20
6
3
6
6
3
3
6
3
3
6
6
6
3
6
3
3
3
3
3
3
6
6
6
3
3
3
3
35
10
50
40
10
80
40
10
10
35
84
40
10
40
10
10
10
10
10
10
24
35
24
10
10
10
10
90
45
85
90
25
60
90
45
45
90
90
90
45
90
45
45
45
45
45
45
90
90
90
45
25
40
45
FluoSpheres
dark red fluorescent microspheres (H2O)
656
FluoSpheres orange
fluorescent microspheres (H2O)
FluoSpheres red fluorescent
microspheres (H2O)
540
576.1
683.1
559.1
607
513.9
521
579
725.1
658
460.9
443.9
670
517.1
564
671.4
551.3
594.5
508.1
505.2
522.9
621.1
650.1
401.1
394.2
659.2
504.9
557.1
FluoSpheres
yellow-green
fluorescent
microspheres
503.1
(H2O)
FluxOR thallium indicator (pH 7.2)
FM 1-43 (lipid)
FM 4-64 (2%
CHAPS)
FxCycle Far Red stain
FxCycle Violet stain
HCS
CellMask Blue stain
HCS CellMask Deep
Red stain
HCS CellMask Green Stain
HCS CellMask Orange stain
492.9
473
505
640.2
359
346.9
649.1
492.8
551.1
Emission
647
Excitation
6
56
Emission
690
Excitation
5
40
Emission
562
Excitation
5
80
Emission
610
Excitation
5
05
Emission
515
Excitation
4
92
Emission
520
Excitation
4
72
Emission
580
Excitation
5
05
Emission
730
Excitation
6
40
Emission
656
Excitation
3
65
Emission
458
Excitation
3
40
Emission
442
Excitation
6
50
Emission
671
Excitation
4
92
Emission
515
Excitation
5
50
Emission
562
3
4
3
3
6
3
3
3
3
3
6
6
3
3
3
3
4
3
3
6
3
4
4
3
3
3
6
10
10
10
10
40
10
10
10
10
10
35
30
10
10
10
10
10
10
10
26
10
10
10
10
10
80
40
45
85
50
45
90
45
45
45
45
45
90
90
45
45
50
45
85
25
45
75
40
85
85
45
45
60
90
-
-
-
-
-
-
-
-
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