研究生科技创新计划 项 目 申 请 书..

的重要底物，细胞骨架蛋白

SSeCKS< /p>

与神经系统的结构和功能关

系密切，但在疼痛模型中的作用和意义 还缺乏系统、深入的研究。本申请课题拟从疼痛的

发生和发展过程，深入研究

及其相互作用分子在疼痛模型中的生理与病理意义。

参考文献

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2004 12

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1

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44-46

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5

二、研究目标、研究内容和拟解决的主要问题

2.1

研究目标：

2.1.1

明确

< br>SSeCKS

与

CCI

损伤，以 及疼痛发生、发展的时空相关性；

2.1.2

明确

SSeCKS

< br>与其相关分子的相互作用在疼痛发生和发展过程中的意义。

2.1.3

明确

< br>SSeCKS

在

PKA

介导的疼 痛传导通路中所起的作用。

2.2

研究内容：

2.2.1

在整体模型水平，

通过构建

CCI

< br>等疼痛模型，

分析

CCI

病程中

SSeCKS

的表达的时空

变化，讨论

SSeCKS

与疼痛生物学行为的可能相关性；在整体模型中干 预

SSeCKS

表达，

对比干预前后各 种指标，进一步探讨

SSeCKS

在其中可能发挥的作用。

2.2.2

在细胞培养水平，