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Beijing University of Chemical Technology
化学工业出版社
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Exercise for Chapter 1
Line 1-10, P. 1
生物工程是属于应用生物科学和技
术的一个领域,
它包含生物或其亚细胞组分在制造
业、服务业和
环境管理等方面的应用。生物工程利用细菌,酵母,真菌,植物细胞或培养的
哺乳动物细
胞作为工业加工的组分。只有将微生物学、生物化学、遗传学、分子生物学、化
学和化学
工程等多种学科和技术结合起来,生物工程的应用才能获得成功。
Biotechnology is an area of applied
bioscience and technology which involves
the
practical application of
biological
organisms,or their
subcellular
components
to
manufacturing
and
service
industries
and
to
environmental
utilizes
bacteria,yeasts
,
fungi
,
alg
ae
,
plant
cells
or
cultured
mammalian
cells
as
constituents
of
industrial application
of
biotechnology
will
result
only
from
the
integration
of
a
multiplicity
of
scientific
disciplines
and
technologies,including <
/p>
microbiology,biochemistry,genetics,molec
ular biology,chemistry and chemical and
process engineering.
line3 ---line7,
生物
科学和生物工程之间有一个很明显的区别。
生物科学对应于
(是
指)
生物知识的
获得,而生物工程强调的是生物知识的应用。在
大部分情况下,生物工程的(加工)过程在
低温下进行,消耗极少的能量,在通常情况下
利用廉价的原料作为底物。
A
clear
distinction
must
be
drawn
between
bioscience
and refers
to
acquisition
of
biological knowledge whereas biotechnology refers
to application of biological
processes
will,in
most
cases,function
at
low
temperatures,will
consume
little
energy and rely in general on
inexpensive raw materials as substrates.
line22---line25,
每个
生物工程的方案都需要持续地评价资源的可获得性,经济效益和对环境的影响,
以及对操
作者和使用者的健康和安全。如果计划得当,生物工程将有助于取得在自然资源,
人类的
需求以及环境之间的平衡。
Each
biotechnological
scenario
will
require
continuous
assessment
of
the
resources
available,the economics and the impact on the
environment,together with
the
health
and
safety
of
operators
and
if
correctly
planned
should
help
to
achieve
a proper balance
between natural resources,human needs and the
environment.
Exercise for Chapter 2
Line 11-17,
新陈代谢是两个紧密联系但又不同
的行为的发源地。
合成代谢过程与形成细胞的材料
有关,这些细
胞材料不仅包括细胞的主要组分(蛋白质,核酸,脂,糖类等)
,还包括它们
的中间前体物
---
氨基酸,嘌呤和嘧啶,脂肪酸
,各种糖和磷酸糖。合成代谢过程不是自发
进行的,
对于大多数
微生物来说,
它们必须由一种能量流来推动,
这种能量流是由“
产生能
量”的分解代谢过程所提供的。
Metabolism
is
a
matrix
of
two
closely
interlinked
but
divergent processes
are
concerned
with
the
building
up
of
cell
materials,not
only
the
major
cell
constituents(proteins,nucleic
acids,lipids,.)but
also
their
intermediate
precursors-amino
acids,purine
and
pyrimidines,fatty
acids,various
sugars
driven
by
an
energy
flow
that
for
most
microorganisms
is
provided
by
a
series
of
“energy
-
yielding”
-catabo
lic processes.
Line 33---line35,
复杂句拆分
Transforming
sugar at the same rate, aerobic yeast gives CO2,
water, and a
relatively
high
yield
of
new
yeast,
whereas
yeast
grown
anaerobically
has
a
relatively
slow
growth
which
is
now
coupled
to
a
high
conversion
of
sugar
into
ethanol
and CO2.
① Transforming
sugar at the same rate, aerobic yeast gives CO2,
water, and a
relatively high yield of
new yeast.
② Yeast grown anaerobically has a
relatively slow growth.
③ It is now
coupled to a high conversion of sugar into ethanol
and CO2.
Line7---11,
复杂句拆分
The
molecular
constitution
of
this
enzyme
is
such
that
its
catalytic
activity
can be modulated
according to the prevailing metabolic
status of
the cell: if more
energy
is
required,
activity
of
PFK
is
increased;
if
the
cell
has
sufficient
energy
or sufficient amounts of C3 metabolites
then the activity of PFK is decreased.
① The molecular
constitution of this enzyme is such.
②
Its
catalytic
activity
can
be
modulated
according
to
the
prevailing
metabolic
status of the cell.
③ More energy
is required.
④ Activity of PFK is
increased.
⑤ The cell has sufficient
energy or sufficient amounts of C3
metabolites.
⑥ The activity of PFK is
decreased.
Exercise for Chapter 3
在生物工程各方面,在通过筛选产
生新生物体方面,人们作了许多的努力,这些新生物体
或者是来源于自然资源,
或者是来自于利用突变或杂交过程
(包括基因工程)
等方法建立的
培养物。为了产生有用的产品,
这些微生物必
需被筛选,
并且它们以相当大的规模生长,以
产生和提取理想产
品,并且要使产品经过严格的评价。
In
all
aspects
of
biotechnology
major
efforts
are
directed
to
screening
programmes
to
generate
new
organisms,either
from
some
natural
source
or
from
established cultures by way of mutation
or by hybridization programmes,including
genetic organisms must be screened for
useful products and grown up on a large
enough scale to produce and extract the
desired product and then to subject the
product to critical evaluation.
Line31-35,
尽管许多新的自养微生物都是野生
型的,但它们都已经从自然环境中被分离出来了,
在运用实验室操作从已经存在的基因组
产生新基因组方面,
人们尽了许多努力。
生物体可以
通过突变、
重组、转化、转导和基因克隆等方法被改变,人们可以使用其中
的一种或几种方
法
(
来改变生物体
p>
)
。
Although
many
new
producer
microorganisms
are
wild-types
and
have
been
isolated
from
natural
environments,major
efforts
are
also
directed
to
generating
new
genomes
from
existing
genomes
by
laboratory
can
be
modified
by
mutation,recombination,tr
ansformation,transduction
and
gene
cloning
either
by
single
processes or in combinations.
Line33-36,
复杂句拆分
In
contrast,
rational
screens
make
more
use
of
the
knowledge
of
the
biochemistry
of
specific
product
formation,
setting
up
a
selection
process
that
uses
a characteristic of the desirable
genotype which is not the particular one of
interest but which can be more readily
scored or assayed.
①
In
contrast,
rational
screens
make
more
use
of
the
knowledge
of
the
biochemistry of specific product
formation, setting up a selection process.
②
The process uses a characteristic of the desirable
genotype.
③ The characteristic is not
the particular one of interest.
④
The characteristic can be more readily scored or
assayed.
?
Exercise for
Chapter 4
Line5-10,
目前
的这些产品的大规模生产过程完全是以前的家庭式生产方式的放大形式。
与这种
产品形式的发展相平行的是人们认识到微生物在去除令人讨厌的垃圾的过程中充当的角色,
这将大规模地影响世界范围内的服务业,包括水的净化,污水处理和垃圾处理。
The
present-day
large
scale
production
processes
of
these
products
are
essentially scaled up
versions of former domestic this development of
product
formation was the recognition
of the role microorganisms could play in removing
unpleasant wastes and this has resulted
in massive world-wide service industries
involved in water purification,effluent
treatment and waste management.
Line31, ,
无论选择什么生物体,
选用什么培养基,
以及生产什么产品,
商业发酵过
程在本质上
都是非常相似的。
在所有的情况下,
大量的具有相同特征的细胞在给定的、
可以控制的条件
下生长。对相同的设备,
只要稍作修改就能用来生产一种酶,
一
种抗生素,
一种有机化学物
质或一种单一的细胞蛋白质。
最简单的发酵过程可以只是把微生物与营养培养基混合,
使其
中的组分发生反应。
The
processes
of
commercial
fermentation
are
in
essence
very
similar
no
matter
what organism is
selected,what medium is used and what product all
cases,large
numbers of cells with
unifom characteristics are grown under
defined,controlled
same
apparatus
with
minor
modifications
can
be
used
to
produce
an
enzyme,an
antibiotic,an
organic chemical or a single cell its simplest
form fermentation
processes
can
be
just
the
mixing
of
microorganisms
with
a
nutrient
broth
and
allowing
the
components to react.
Line25-31,
<
/p>
然而,
对于许多分批培养来说,
指数生长
是短暂的。
当营养物被生长的细胞种群用完
时,
无限制的生长将会被有限的生长所代替,
尽管细胞的数量还在增加,
而在特定点的比生
长速率则趋向于越来越小,也就是说:小于
μmax。降速期之后是稳定期,在此期间(在这
个时期)
< br>,由于营养物的耗尽,整个生长将不再进行。
However,in most
batch cultivations exponential growth is
nutrients are used
up
by
the
growing
cell
population
unlimited
growth
will
be
replaced
by
limited
growth
and although the cell population is
still increasing the specific growth rate at
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