-actor
提取方法
2
：
Zhang CF, Peng SB, Peng XX,
Chavez AQ,Bennett J. Response of
glutamine synthetase isoforms to
nitrogen sources in rice
Oryza
Sativa
.L
roots.
Plant
Sci
,1997,125:163-170
Rhodes
D,
Rendo
GA,
Stewart
GR.
The
control
of
glutamine
synthetase
level in
Lemna minor
L. Planta, 1975,
125:201-211
The reaction
mixture (3.0 ml) consisted
of sodium
ketoglutarate (10_mol), ammonium chloride
(10_mol), calcium chloride (10_mol),
NADH (2_mol), Tris
–
HCl
buffer (100_mol, pH 8.2) and enzyme 0.1
ml.
Following a 30 min
incubation period at 30
?
C in
20
μ
mol
MgSO
4
,
80
μ
mol
α
-ketoglutarate,
6
μ
mol
hydroxylamine,
8
μ
mol ATP and enzyme
extract, the enzymatic reaction was terminated by
the
addition of 1.5 ml of a solution
containing 0.37mol/l FeCl
3
,
0.2 mol/l TCA and 0.37 mol/l HCl and
the amount of glutamyl hydroxamate
determined at 540 nm.
Lea P
J, Blackwell R D, Chen F L. Enzymes of primary
metabolism. In: Harborne J B.
Methods
in Plant Biochemistry. Vol.3. New York: Academic
Press, 1990. pp 260-273